An aptamer-based colorimetric lead(II) assay based on the use of gold nanoparticles modified with dsDNA and exonuclease I

The authors describe a colorimetric method for the sensitive and selective detection of Pb(II). It is based on the use exonuclease I (Exo I), a Pb(II)-binding aptamer bound to gold nanoparticles (AuNPs), and a DNA strand that complementary to the aptamer. In the absence of Pb(II), the dsDNA on the AuNPs prevents aggregation of the AuNPs in the presence of NaCl. In the presence of Pb(II), however, the aptamer binds Pb(II) and complementary strand is released and digested by Exo I. As a result, the solution of AuNPs undergoes a color change from red to purple if salt is added to the sample. The assay is selective for Pb(II) and has a limit of detection as low as 2.4 nM. It was successfully applied to the determination of Pb(II) in spiked tap water.