Mapping of Intracellular Concentrations of Macromolecules by Two-photon Excited Fluorescence Lifetime Imaging

被引：3

作者：

Liu, Lixin ^{[1
]}

Pliss, Artem ^{[2
,3
]}

Peng, Xiao ^{[4
]}

Kuzmin, Andrey ^{[2
,3
]}

Qu, Junle ^{[4
]}

Prasad, Paras N. ^{[2
,3
]}

机构：

[1] Xidian Univ, Sch Phys & Optoelect Engn, Xian 710071, Peoples R China

[2] Univ Buffalo State Univ New York, Inst Lasers Photon & Biophoton, Buffalo, NY 14260 USA

[3] Univ Buffalo State Univ New York, Dept Chem, Buffalo, NY 14260 USA

[4] Shenzhen Univ, Coll Optoelect Engn, Minist Educ & Guangdong Prov, Key Lab Optoelect Devices & Syst, Shenzhen 518060, Peoples R China

来源：

MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES XVI | 2016年 / 9712卷

关键词：

fluorescence lifetime imaging microscopy (FLIM); two-photon excited fluorescence; refractive index; protein concentration; macromolecule; REFRACTIVE-INDEX; PROTEINS; ENVIRONMENT; BODIES; DECAY;

D O I：

10.1117/12.2211889

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Measurements and monitoring of concentrations of macromolecules in live cells and sub-cellular structures is of tremendous interest in cell biology and translational medicine. In this report we demonstrate a breakthrough potential of FLIM for real-time quantitative mapping of macromolecular distribution in the cell. In our approach we exploit a correlation existing between the fluorescence lifetime of fluorophores, refractive index and local concentrations of cellular macromolecules in the of fluorophore's microenvironment. We show a value of our approach for fundamental cell science and cellular diagnostic assays.

引用

页数：8

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