The clock components Period2, Cryptochrome1a, and Cryptochrome2a function in establishing light-dependent behavioral rhythms and/or total activity levels in zebrafish

被引：19

作者：

Hirayama, Jun ^{[1
,2
]}

Alifu, Yikelamu ^{[1
]}

Hamabe, Rin ^{[1
]}

Yamaguchi, Sho ^{[3
]}

Tomita, Jun ^{[3
]}

Maruyama, Yusuke ^{[4
]}

Asaoka, Yoichi ^{[5
]}

Nakahama, Ken-ichi ^{[6
]}

Tamaru, Teruya ^{[7
,8
]}

Takamatsu, Ken ^{[7
,8
]}

Takamatsu, Nobuhiko ^{[9
]}

Hattori, Atsuhiko ^{[4
]}

Nishina, Sachiko ^{[10
,11
]}

Azuma, Noriyuki ^{[10
,11
]}

Kawahara, Atsuo ^{[12
]}

Kume, Kazuhiko ^{[3
]}

Nishina, Hiroshi ^{[1
]}

机构：

[1] TMDU, Med Res Inst, Dept Dev & Regenerat Biol, Tokyo, Japan

[2] Komatsu Univ, Fac Hlth Sci, Dept Clin Engn, Ishikawa, Japan

[3] Nagoya City Univ, Grad Sch Pharmaceut Sci, Dept Neuropharmacol, Nagoya, Aichi, Japan

[4] TMDU, Dept Biol, Coll Liberal Arts & Sci, Ichikawa, Japan

[5] Yamaguchi Univ, Grad Sch Med, Dept Microbiol & Immunol, Ube, Yamaguchi, Japan

[6] TMDU, Dept Cellular Physiol Chem, Tokyo, Japan

[7] Toho Univ, Sch Med, Dept Physiol, Tokyo, Japan

[8] Toho Univ, Sch Med, Adv Res Ctr Med Sci, Tokyo, Japan

[9] Kitasato Univ, Sch Sci, Dept Biosci, Sagamihara, Kanagawa, Japan

[10] Natl Ctr Child Hlth & Dev, Dept Ophthalmol, Tokyo, Japan

[11] Natl Ctr Child Hlth & Dev, Lab Visual Sci, Tokyo, Japan

[12] Univ Yamanashi, Grad Sch Med Sci, Ctr Med Educ & Sci, Lab Dev Biol, Yamanashi, Japan

来源：

SCIENTIFIC REPORTS | 2019年 / 9卷 / 1期

基金：

日本学术振兴会;

关键词：

CIRCADIAN-RHYTHMS; LIPID-METABOLISM; GENE; CELL; EXPRESSION; SYSTEM; SLEEP; TRANSCRIPTION; MELATONIN; PATHWAYS;

D O I：

10.1038/s41598-018-37879-8

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The circadian clock generates behavioral rhythms to maximize an organism's physiological efficiency. Light induces the formation of these rhythms by synchronizing cellular clocks. In zebrafish, the circadian clock components Period2 (zPER2) and Cryptochrome1a (zCRY1a) are light-inducible, however their physiological functions are unclear. Here, we investigated the roles of zPER2 and zCRY1a in regulating locomotor activity and behavioral rhythms. zPer2/zCry1a double knockout (DKO) zebrafish displayed defects in total locomotor activity and in forming behavioral rhythms when briefly exposed to light for 3-h. Exposing DKO zebrafish to 12-h light improved behavioral rhythm formation, but not total activity. Our data suggest that the light-inducible circadian clock regulator zCRY2a supports rhythmicity in DKO animals exposed to 12-h light. Single cell imaging analysis revealed that zPER2, zCRY1a, and zCRY2a function in synchronizing cellular clocks. Furthermore, microarray analysis of DKO zebrafish showed aberrant expression of genes involved regulating cellular metabolism, including ATP production. Overall, our results suggest that zPER2, zCRY1a and zCRY2a help to synchronize cellular clocks in a light-dependent manner, thus contributing to behavioral rhythm formation in zebrafish. Further, zPER2 and zCRY1a regulate total physical activity, likely via regulating cellular energy metabolism. Therefore, these circadian clock components regulate the rhythmicity and amount of locomotor behavior.

引用

页数：15

共 63 条

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