Structural characterization, modification and hepatoprotective effects of polysaccharide from Mori Fructus

被引：42

作者：

Deng, Qingfang ^{[1
,2
,3
]}

Wang, Xing ^{[1
,2
,3
]}

Chen, Huaguo ^{[1
,2
,3
]}

Zhao, Chao ^{[1
,2
,3
]}

Gong, Xiaojian ^{[1
,2
,3
]}

Zhou, Xin ^{[1
,2
,3
]}

机构：

[1] Guizhou Normal Univ, Key Lab Informat Syst Mt Areas & Protect Ecol Env, Guiyang 550001, Guizhou, Peoples R China

[2] Guizhou Normal Univ, Guizhou Engn Lab Qual Control & Evaluat Technol M, Guiyang 550001, Guizhou, Peoples R China

[3] Guizhou Normal Univ, Res Ctr Qual Control Nat Med, Guiyang 550001, Guizhou, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2020年 / 153卷

基金：

中国国家自然科学基金;

关键词：

Mori Fructus polysaccharide; Structure modification; Hepatoprotective effects; SULFATED MODIFICATION; ANTIOXIDANT; CARBOXYMETHYLATION; ANTITUMOR; EXTRACTION;

D O I：

10.1016/j.ijbiomac.2020.02.300

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this paper, three purified Mori Fnictus (M. Fructus) polysaccharides (MFP-1, MFP-2 and MR-3) were successfully obtained from M. Fnictus. Structural modification methods, including oxidative degradated, sulfated and carboxymethylated modification, were conducted and nine MFPs derivatives were obtained. The molecular structures of MFPs were studied and the results showed that MFP-1, MFP-2 and MFP-3, mainly composed of Ara, Gal, Rha, Glc and GalA, with a molecular weight approximately of 20.93 kDa, 5623 kDa and 118.76 kDa, respectively. FT-IR spectrum of these MFPs all revealed the characteristic polysaccharide absorption bands, while the MFPs derivatives also exhibited some differences due to the structure modification. Hepatoprotective activities tests showed that MFP derivatives could significantly improve the alcohol dehydrogenase (ADH) activity by comparing with the native MFP. And among them, the C-MFP exhibited higher protection effect than the other two modification derivatives. (C) 2020 Published by Elsevier B.V.

引用

页码：357 / 363

页数：7

共 28 条

[1] Sulfated polysaccharides from common smooth hound: Extraction and assessment of anti-ACE, antioxidant and antibacterial activities [J].