Magnetic porous corn starch for the affinity purification of cyclodextrin glucanotransferase produced by Bacillus circulans

被引：2

作者：

Mirka, Safarikova ^{[1
]}

Katerina, Horska ^{[1
]}

Zdenka, Maderova ^{[1
]}

Alexandra, Tonkova ^{[2
]}

Viara, Ivanova-Pashkoulova ^{[3
]}

Ivo, Safarik ^{[1
]}

机构：

[1] GCRC AS CR, Dept Nanobiotechnol, Inst Nanobiol & Struct Biol, Ceske Budejovice 37005, Czech Republic

[2] Inst Microbiol BAS, Dept Extremophil Bacteria, Sofia, Bulgaria

[3] Univ Food Technol, Dept Organ Chem & Microbiol, Plovdiv, Bulgaria

来源：

BIOCATALYSIS AND BIOTRANSFORMATION | 2012年 / 30卷 / 01期

关键词：

Bacillus circulans; cyclodextrin glucanotransferase; magnetic starch; magnetic separation; GLYCOSYLTRANSFERASE; ATCC-21783; ALKALINE; GEL;

D O I：

10.3109/10242422.2012.646665

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Magnetic porous corn starch was prepared as an affinity adsorbent for the efficient and simple scale-up procedure for one-step purification of cyclodextrin glucanotransferase (CGTase) from Bacillus circulans. Magnetic affinity separation enabled isolation of CGTase from cultivation media (volumes between 10 and 400 mL) with ca 60-70% recovery after elution with alkaline buffers containing soluble starch; the enzyme purification factor was 19-25 in different batches. The majority of ballast proteins were removed during the purification process, which shows high selectivity of the affinity material

引用

页码：96 / 101

页数：6

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