A Genosensor for Point Mutation Detection of P53 Gene PCR Product Using Magnetic Particles

被引：39

作者：

Hamidi-Asl, Ezat ^{[1
,2
]}

Raoof, Jahan Bakhsh ^{[2
]}

Hejazi, Mohammad Saeid ^{[3
,4
,5
]}

Sharifi, Simin ^{[3
]}

Golabi, Seyed Mahdi ^{[6
]}

Palchetti, Ilaria ^{[1
,7
]}

Mascini, Marco ^{[7
]}

机构：

[1] Univ Florence, Dipartimento Chim, I-50019 Sesto Fiorentino, Italy

[2] Univ Mazandaran, Electroanalyt Chem Res Lab, Dept Analyt Chem, Fac Chem, Babol Sar, Iran

[3] Tabriz Univ Med Sci, Fac Pharm, Tabriz, Iran

[4] Tabriz Univ Med Sci, Drug Appl Res Ctr, Tabriz, Iran

[5] Tabriz Univ Med Sci, Pharmaceut Nanotechnol Res Ctr, Tabriz, Iran

[6] Tabriz Univ, Electanalyt Chem Res Lab, Dept Analyt Chem, Fac Chem, Tabriz, Iran

[7] Ist Nazl Biostrut & Biosistemi, I-00136 Rome, Italy

来源：

ELECTROANALYSIS | 2015年 / 27卷 / 06期

关键词：

Magnetic beads; ELISA; Sandwich hybridization; Microfluidic platform; PCR products; ELECTROCHEMICAL DNA; TUMOR-SUPPRESSOR; PASTE ELECTRODE; PNA BIOSENSOR; BEADS; ASSAY; OLIGONUCLEOTIDE; INDICATOR; SEQUENCE; COMPLEX;

D O I：

10.1002/elan.201400660

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The development of an electrochemical genosensor involving DNA biotinylated capture probe immobilized on streptavidin coated paramagnetic beads and microfluidic based platform for the detection of P53 gene PCR product is reported. The novelty of this work is the combination of a sensitive electrochemical platform and a proper microfluidic system with a simple and effective enzyme signal amplification technology, ELISA, for detection of target DNA sequence and single nucleotide mutation in p53 tumor suppressor gene sequence. The biosensor has been applied to detect the PCR amplified samples and the results shows that it can discriminate successfully perfect matched DNA from mutant form.

引用

页码：1378 / 1386

页数：9

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