Live attenuated pseudorabies virus developed using the CRISPR/Cas9 system

被引：55

作者：

Tang, Yan-Dong ^{[1
]}

Liu, Ji-Ting ^{[1
,2
]}

Wang, Tong-Yun ^{[1
]}

An, Tong-Qing ^{[1
]}

Sun, Ming-Xia ^{[1
]}

Wang, Shu-Jie ^{[1
]}

Fang, Qiong-Qiong ^{[1
]}

Hou, Lin-lin ^{[1
]}

Tian, Zhi-Jun ^{[1
]}

Cai, Xue-Hui ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, State Key Lab Vet Biotechnol, Harbin Vet Res Inst, Harbin 150001, Peoples R China

[2] Jilin Agr Univ, Coll Anim Sci & Technol, Changchun 130018, Peoples R China

来源：

VIRUS RESEARCH | 2016年 / 225卷

基金：

美国国家科学基金会;

关键词：

CRISPR/Cas9; Pseudorabies virus; Attenuation; Vaccine; GENOMIC CHARACTERIZATION; HUMAN-CELLS; VARIANT; CHINA; PATHOGENICITY; SWINE; CAS9;

D O I：

10.1016/j.virusres.2016.09.004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Currently, pseudorabies virus (PRV) variant strains are outbreaking in China; these variants belong to genotype II PRV. The traditional Bartha-K61 vaccine has failed to provide complete protection against the emergent variant strains. Therefore, rapid attenuation of current epidemic strains is needed for effective PRV control. In this study, we report a rapid method for editing the PRV genome using the CRISPR-Cas9 system. We developed a triple gE/gI/TK gene-inactivated HeN1 PRV strain, because mice were more susceptible to PRV infection, we then evaluated the attenuation of PRV in the mice and demonstrated that modified PRV was fully attenuated. Furthermore, the attenuated strain also induced immune protection in response to a parental PRV challenge. Overall, we showed that PRVs can be rapidly attenuated using CRISPR-Cas9 technology, which will be critical for PRV control, especially when new variant PRV strains emerge. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：33 / 39

页数：7

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