Functional silencing of hepatic microsomal glucose-6-phosphatase gene expression in vivo by adenovirus-mediated delivery of short hairpin RNA

被引：12

作者：

Huang, A ^{[1
]}

Chen, Y ^{[1
]}

Wang, XZ ^{[1
]}

Zhao, SC ^{[1
]}

Su, N ^{[1
]}

White, DW ^{[1
]}

机构：

[1] Millennium Pharmaceut, Cambridge, MA 02139 USA

来源：

FEBS LETTERS | 2004年 / 558卷 / 1-3期

关键词：

RNA interference; short hairpin RNA; gene silencing; glucose-6-phosphatase; adenovirus;

D O I：

10.1016/S0014-5793(03)01499-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An expression cassette containing mouse U6 polymerase III promoter directing expression of short hairpin RNA (shRNA) targeting murine microsomal glucose-6-phosphatase (G6P) transcript was generated. This construct was packaged into an adenoviral (AdV) backbone and viral stocks generated. Mice injected intravenously with AdV-G6PshRNA exhibited a significant reduction in postprandial glucose levels and had significantly elevated steady-state hepatic glycogen stores. Target gene silencing was confirmed by measurements demonstrating a significant reduction in both hepatic G6P transcript level and phosphohydrolase activity. These findings provide evidence that AdV delivery of expressed shRNA can be a productive tool to explore gene function in vivo. (C) 2003 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：69 / 73

页数：5

共 29 条

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