Single-cell epigenetics - Chromatin modification atlas unveiled by mass cytometry

被引：24

作者：

Cheung, Peggie ^{[1
,2
]}

Vallania, Francesco ^{[1
,3
]}

Dvorak, Mai ^{[1
,2
]}

Chang, Sarah E. ^{[1
,2
]}

Schaffert, Steven ^{[1
,3
]}

Donato, Michele ^{[1
,3
]}

Rao, Aditya M. ^{[1
,3
]}

Mao, Rong ^{[1
,2
]}

Utz, Paul J. ^{[1
,2
]}

Khatri, Purvesh ^{[1
,3
]}

Kuo, Alex J. ^{[1
,2
]}

机构：

[1] Stanford Univ, Sch Med, Inst Immun Transplantat & Infect, Stanford, CA 94305 USA

[2] Stanford Univ, Sch Med, Div Immunol & Rheumatol, Dept Med, Stanford, CA 94305 USA

[3] Stanford Univ, Sch Med, Div Biomed Informat Res, Dept Med, Stanford, CA 94305 USA

来源：

CLINICAL IMMUNOLOGY | 2018年 / 196卷

基金：

比尔及梅琳达.盖茨基金会;

关键词：

Epigenetics; Mass cytometry; EpiTOF; Chromatin; Histone post-translational modifications; Immune system; HISTONE H3; LYSINE; 36; PHOSPHORYLATION; BINDING; ACCESSIBILITY; PROTEINS; VARIANTS; IMMUNE; H2B;

D O I：

10.1016/j.clim.2018.06.009

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Modifications of histone proteins are fundamental to the regulation of epigenetic phenotypes. Dysregulations of histone modifications have been linked to the pathogenesis of diverse human diseases. However, identifying differential histone modifications in patients with immune-mediated diseases has been challenging, in part due to the lack of a powerful analytic platform to study histone modifications in the complex human immune system. We recently developed a highly multiplexed platform, Epigenetic landscape profiling using cytometry by Time-Of-Flight (EpiTOF), to analyze the global levels of a broad array of histone modifications in single cells using mass cytometry. In this review, we summarize the development of EpiTOF and discuss its potential applications in biomedical research. We anticipate that this platform will provide new insights into the roles of epigenetic regulation in hematopoiesis, immune cell functions, and immune system aging, and reveal aberrant epigenetic patterns associated with immune-mediated diseases.

引用

页码：40 / 48

页数：9

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