Structure and mapping of the human β-defensin HBD-2 gene and its expression at sites of inflammation

被引：222

作者：

Liu, L

Wang, LN

Jia, HP

Zhao, CQ

Heng, HHQ

Schutte, BC

McCray, PB

Ganz, T

机构：

[1] Univ Calif Los Angeles, Sch Med, Dept Med, Los Angeles, CA 90095 USA

[2] Univ Calif Los Angeles, Sch Med, Dept Pathol, Los Angeles, CA 90095 USA

[3] Univ Calif Los Angeles, Sch Med, Will Rogers Inst Pulm Res, Los Angeles, CA 90095 USA

[4] Harbor UCLA Med Ctr, Dept Pathol, Los Angeles, CA USA

[5] Univ Iowa, Dept Pediat, Iowa City, IA 52242 USA

[6] York Univ, Dept Biol, N York, ON M3J 1P3, Canada

[7] SeeDNABiotech Inc, N York, ON M3J 1P3, Canada

来源：

GENE | 1998年 / 222卷 / 02期

关键词：

host defense; epithelia; chromosome; 8p23; antimicrobial peptides;

D O I：

10.1016/S0378-1119(98)00480-6

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We cloned a second human beta-defensin gene, HBD-2, and determined its gene structure and expression in inflamed tissue sections. The entire gene spanned about 2 kb with two small exons and one intron. Radiation hybrid studies confirmed the location on chromosome 8p, were consistent with the order HNP-1, HBD-1 and HBD-2, and located HBD-2 as the most centromeric of the genes. By three-color fluorescence in situ hybridization on both free chromatin fiber mapping and interphase mapping, HBD-1, HBD-2 and HNP-1 were mapped to chromosome 8p23. HBD-1 was within 40-100 kb of HNP-1, while HBD-2 was about 500-600 kb from HBD-1, with the most likely order HNP-1, HBD-1, HBD-2. The expression of HBD-2 was locally regulated by inflammation. HBD-2 mRNA was markedly increased in the epidermis surrounding inflamed regions, but not detectable in adjacent non-inflamed areas, a distribution that was confirmed at the peptide level by immunostaining with HBD-2 antibody. The HBD-2 gene is the first member of the human defensin family that is locally inducible by inflammation. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：237 / 244

页数：8