Enhancing production of ergosterol in Pichia pastoris GS115 by over-expression of 3-hydroxy-3-methylglutaryl CoA reductase from Glycyrrhiza uralensis

被引：9

作者：

Liu, Ying ^{[1
]}

Zhu, Xiaoqing ^{[1
]}

Li, Wendong ^{[2
]}

Wen, Hao ^{[1
]}

Gao, Ya ^{[1
]}

Liu, Yong ^{[1
]}

Liu, Chunsheng ^{[1
]}

机构：

[1] Beijing Univ Chinese Med, Sch Chinese Pharm, Beijing 100102, Peoples R China

[2] Beijing Inst Drug Control, Dept Antibiot, Beijing 100102, Peoples R China

来源：

ACTA PHARMACEUTICA SINICA B | 2014年 / 4卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Glycyrrhiza uralensis Fisch; 3-Hydroxy-3-methylglutaryl-CoA reductase gene; Over-expression; Pichia pastoris; Copy number variation;

D O I：

10.1016/j.apsb.2014.02.007

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The rate-limiting enzyme in the mevalonic acid (MVA) pathway which can lead to triterpenoid saponin glycyrrhizic acid (GA) is 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR). In order to reveal the effect of copy number variation in the HMGR gene on the MVA pathway, the HMGR gene from Glycyrrhiza uralensis Fisch. (GuHMGR) was cloned and over-expressed in Pichia pastoris GS115. Six recombinant P. pastoris strains containing different copy numbers of the GuHMGR gene were obtained and the content of ergosterol was analyzed by HPLC. The results showed that all the recombinant P. pastoris strains contained more ergosterol than the negative control and the strains with 8 and 44 copies contained significantly more ergosterol than the other strains. However, as the copy number increased, the content of ergosterol showed an increasing-decreasing-increasing pattern. This study provides a rationale for increasing the content of GA through over-expressing the GuHMGR gene in cultivars of G. uralensis. (C) 2014 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences.

引用

页码：161 / 166

页数：6

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