Evaluation for anti-inflammatory effects of Siegesbeckia glabrescens extract in vitro

被引：10

作者：

Lee, J. H. ^{[1
]}

Kang, B. S. ^{[2
]}

Hwang, K. H. ^{[1
]}

Kim, G. H. ^{[1
]}

机构：

[1] Duksung Womens Univ, Plant Resources Res Inst, Seoul 132714, South Korea

[2] Cheonan Yonam Coll Univ, Dept Ecofriendly Hort, Cheonan 331709, South Korea

来源：

FOOD AND AGRICULTURAL IMMUNOLOGY | 2011年 / 22卷 / 02期

关键词：

Siegesbeckia glabrescens; anti-inflammation; antioxidant activity; PGE(2); COX-2; NITRIC-OXIDE SYNTHASE; RAW; 264.7; CELLS; KAPPA-B; CYCLOOXYGENASE-2; INTERLEUKIN-1-BETA; FIBROBLASTS; ANTIOXIDANT; SUPPRESSION; INHIBITION; CYTOKINES;

D O I：

10.1080/09540105.2010.549210

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The aim of this study is to elucidate the anti-inflammatory effects of Siegesbeckia glabrescens extract in vitro. The n-butanol fraction (100 mu g/ml) of S. glabrescens had an approximately 92% inhibitory effect on nitrite production from lipopolysaccharide (LPS)-induced macrophage cells, whereas other fractions showed relatively low nitric oxide (NO) inhibition. The n-butanol and ethyl acetate fractions of S. glabrescens showed significant chemical NO quenching to approximately 39-46% in the cell-free system, and showed the decreases of inducible nitric oxide synthase (iNOS) transcription potently. The hexane, n-butanol, and ethyl acetate fractions of S. glabrescens showed approximately 90-96% inhibition of prostaglandin E-2 (PGE(2)) synthesis. The ethyl acetate, n-butanol, and aqueous fractions of S. glabrescens showed a potent inhibition of LPS-induced cyclooxygenase-2 (COX-2) mRNA transcription. All S. glabrescens extract-fractions showed significant decreases of IL-1 beta biosynthesis to 69.7-97.0%, as compared to LPS-induced IL-1 beta production in macrophage cells.

引用

页码：145 / 160

页数：16

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