Gradient-enhanced one-dimensional heteronuclear NOE experiment with H-1 detection

A new experiment, HETGOESY, is described in which C-13-H-1 NOE enhancements are detected directly on H-1 The experiment uses pulsed field gradients for coherence selection, thus completely avoiding the problems associated with difference spectroscopy. It is shown, both theoretically and experimentally, that the new experiment has sensitivity advantages over the conventional C-13{H-1} steady-state NOE difference experiment. The new experiment also has the advantage that it uses selective excitation in the generally better resolved decoupled C-13 spectrum rather than the H-1 spectrum.