Amperometric Immunosensor for Carcinoembryonic Antigen Based on Poly(2,6-pyridinediamine) Membrane and Gold Nanoparticles

被引：0

作者：

Guan Shu ^{[1
]}

Yuan Ruo ^{[1
]}

Chai Yaqin ^{[1
]}

Tang Dianping ^{[1
]}

Liu Kaige ^{[1
]}

Wang Jinfen ^{[1
]}

机构：

[1] SW China Univ, Coll Chem Engn, Key Lab Luminescence & Real Time Anal MOE, Chongqing 400715, Peoples R China

来源：

ACTA CHIMICA SINICA | 2009年 / 67卷 / 16期

关键词：

immunosensor; poly(2,6-pyridinediamine); thionine; gold nanoparticle; carcinoembryonic antigen; DNA HYBRIDIZATION; THIONINE; POLYPYRROLE; FILMS; HRP;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The poly 2,6-pyridinediamine (pPA) film was formed on the glassy carbon electrode by utilizing electropolymerization, which yielded an interface with amine groups for the assembly of thionine (Thi) and colloidal Au. The electrochemical characteristics of the immunosensor were studied by cyclic voltammetry. The factors influencing the performance of the resulting biosensor were studied in detail. Under the optimized conditions, the current response of the proposed immunosensor decreased linearly in two concentration ranges of CEA from 0.5 to 10 ng/mL and from 10 to 120 ng/mL with a detection limit of 0.2 ng/mL. Moreover, the studied immunosensor exhibited simple fabrication, good reproducibility and a broad linear range, which made it potentially application for determination of CEA in clinical analysis.

引用

页码：1923 / 1928

页数：6

共 25 条

[1] Immune responses in cancer [J].