Role of annexin A2 in cellular entry of rabbit vesivirus

被引：27

作者：

Gonzalez-Reyes, Salome ^{[1
]}

Garcia-Manso, Alberto ^{[1
]}

del Barrio, Gloria ^{[1
]}

Dalton, Kevin P. ^{[1
]}

Gonzalez-Molleda, Lorenzo ^{[1
]}

Arrojo-Fernandez, Jose ^{[1
]}

Nicieza, Ines ^{[1
]}

Parra, Francisco ^{[1
]}

机构：

[1] Univ Oviedo, Inst Univ Biotecnol Asturias, Dept Bioquim & Biol Mol, E-33006 Oviedo, Spain

来源：

JOURNAL OF GENERAL VIROLOGY | 2009年 / 90卷

关键词：

INFECTIOUS NONBACTERIAL GASTROENTERITIS; LYMPHOCYTIC CHORIOMENINGITIS VIRUS; BLOOD GROUP ANTIGENS; FELINE-CALICIVIRUS; MASS-SPECTROMETRY; HUMAN CYTOMEGALOVIRUS; MEMBRANE-PROTEIN; EPITHELIAL-CELLS; MAMMALIAN-CELLS; RECEPTOR;

D O I：

10.1099/vir.0.013276-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The mechanisms of calicivirus attachment and internalization are not well understood, mainly due to the lack of a reliable cell-culture system for most of its members. In this study, rabbit vesivirus (RaV) virions were shown to bind annexin A2 (ANXA2) in a membrane protein fraction from HEK293T cells, using a virus overlay protein-binding assay and matrix-assisted laser desorption/ionization time-of-flight analysis. A monoclonal anti-ANXA2 antibody and small interfering RNA-mediated knockdown of ANXA2 expression in HEK293T cells reduced virus infection significantly, further supporting the role of ANXA2 in RaV attachment and/or internalization.

引用

页码：2724 / 2730

页数：7

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