Expression of the bovine leukemia virus envelope glycoprotein (gp51) by recombinant baculovirus and its use in an enzyme-linked immunosorbent assay

被引：22

作者：

De Giuseppe, A ^{[1
]}

Feliziani, F ^{[1
]}

Rutili, D ^{[1
]}

De Mia, GM ^{[1
]}

机构：

[1] Ist Zooprofilatt Sperimentale Umbria Marche, I-06126 Perugia, Italy

来源：

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY | 2004年 / 11卷 / 01期

关键词：

D O I：

10.1128/CDLI.11.1.147-151.2004

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The gene encoding the major envelope glycoprotein (gp51) with its signal sequence, represented by an additional NH2-terminal 33-residue amino acid sequence of bovine leukemia virus (BLV), was inserted into a baculovirus transfer vector. A recombinant virus expressing a secreted gp51 protein in insect cells was isolated. The recombinant gp51 expressed was characterized by using an anti-BLV monoclonal antibody by both Western blotting analysis and enzyme-linked immunosorbent assay (ELISA). The secreted gp51 was used as an antigen, and an ELISA with recombinant gp51 (rgp51) was developed for the detection of BLV antibodies. This new procedure was compared with a previous ELISA method for the detection of BLV antibodies and an agar gel immunodiffusion test performed with an unpurified BLV antigen preparation. The comparative testing of field samples showed that the ELISA with rgp51 is more specific and also suitable for the testing of pooled sera.

引用

页码：147 / 151

页数：5

共 26 条

[1] ENVELOPE GLYCOPROTEIN-GP51 OF BOVINE LEUKEMIA-VIRUS IS DIFFERENTLY GLYCOSYLATED IN CELLS OF VARIOUS SPECIES AND ORGAN ORIGIN [J].

ALTANER, C ;

MERZA, M ;

ALTANEROVA, V ;

MOREIN, B .

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 1993, 36 (02) :163-177

[2] MAPPING OF SEQUENTIAL EPITOPES RECOGNIZED BY MONOCLONAL-ANTIBODIES ON THE BOVINE LEUKEMIA-VIRUS EXTERNAL GLYCOPROTEINS EXPRESSED IN ESCHERICHIA-COLI BY MEANS OF ANTIPEPTIDE ANTIBODIES [J].

BAN, J ;

CZENE, S ;

ALTANER, C ;

CALLEBAUT, I ;

KRCHNAK, V ;

MERZA, M ;

BURNY, A ;

KETTMANN, R ;

PORTETELLE, D .

JOURNAL OF GENERAL VIROLOGY, 1992, 73 :2457-2461

[3]

BEX F, 1979, CANCER RES, V39, P1118

[4] EPITOPES OF BOVINE LEUKEMIA-VIRUS GLYCOPROTEIN GP51 RECOGNIZED BY SERA OF INFECTED CATTLE AND SHEEP [J].

BRUCK, C ;

PORTETELLE, D ;

MAMMERICKX, M ;

MATHOT, S ;

BURNY, A .

LEUKEMIA RESEARCH, 1984, 8 (03) :315-321

[5]

Burny A., 1990, Retrovirus biology and human disease., P9

[6]

*COUNC EUR COMM, 1984, DIRECTIVE 84 463 CEE

[7]

DEMIA GM, 1991, ATTI SOC ITAL SCI VE, V44, P863

[8] AN INTERNATIONAL COMPARISON OF DIFFERENT LABORATORY TESTS FOR THE DIAGNOSIS OF BOVINE LEUKOSIS - SUGGESTIONS FOR INTERNATIONAL STANDARDIZATION [J].

HOFFJORGENSEN, R .

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 1989, 22 (03) :293-297

[9] An effective peptide vaccine to eliminate bovine leukaemia virus (BLV) infested cells in carrier sheep [J].

Kabeya, H ;

Ohashi, K ;

Ohishi, K ;

Sugimoto, C ;

Amanuma, H ;

Onuma, M .

VACCINE, 1996, 14 (12) :1118-1122

[10] EXPRESSION OF BOVINE LEUKEMIA-VIRUS ENVELOPE GENE BY RECOMBINANT VACCINIA VIRUSES [J].

KUMAR, S ;

ANDREW, ME ;

BOYLE, DB ;

BRANDON, RB ;

LAVIN, MF ;

DANIEL, RCW .

VIRUS RESEARCH, 1990, 17 (02) :131-142

← 1 2 3 →