Catalytic effect of transglutaminase mediated by myofibrillar protein crosslinking under microwave irradiation

被引：43

作者：

Cao, Hongwei ^{[1
,2
,3
,4
]}

Jiao, Xidong ^{[2
,3
,4
]}

Fan, Daming ^{[1
,2
,3
,4
]}

Huang, Jianlian ^{[1
,5
]}

Zhao, Jianxin ^{[2
,3
,4
]}

Yan, Bowen ^{[2
,3
,4
]}

Zhou, Wenguo ^{[1
,5
]}

Zhang, Wenhai ^{[1
,5
]}

Ye, Weijian ^{[1
,5
]}

Zhang, Hao ^{[2
,3
,4
]}

机构：

[1] Minist Agr & Rural Affairs, Key Lab Refrigerat & Conditioning Aquat Prod Proc, Xiamen 361022, Peoples R China

[2] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China

[3] Jiangnan Univ, Natl Engn Res Ctr Funct Food, Wuxi 214122, Peoples R China

[4] Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Peoples R China

[5] Fujian Anjoyfood Share Co Ltd, Xiamen 361022, Peoples R China

来源：

FOOD CHEMISTRY | 2019年 / 284卷

基金：

中国国家自然科学基金;

关键词：

Microwave; Myofibrillar protein; Tranglutaminase; Crosslinking; Gel property; Secondary structure; SECONDARY STRUCTURE; ALASKA POLLOCK; GEL PROPERTIES; MECHANISM; GELATION; OXIDASE; PATTERN; MYOSIN; ACID; PORK;

D O I：

10.1016/j.foodchem.2019.01.097

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Microwave (MW) heating improved the activity of transglutaminase (TGase) by inducing conformational changes due to structural modification. However, when TGase and myofibrillar protein were heated, the solubility and degree of crosslinking were similar. Further, the gel properties of the mixed solution pre-gelled by MW heating were lower than that obtained with water bath (WB) pre-gelling. We compared the effects on myofibrillar proteins at the same heating rate, our results showed that MW promoted aggregation, as the particle distribution tended toward larger molecular size. The increase of random coil as investigated by circular dichroism (CD) indicated that WB induced the unfolding of myofibrillar protein. MW enhanced intermolecular forces by engendering more disulfide bonds, which hindered the catalysis by TGase. Finally, SDS-PAGE indicated that the myosin molecules had more head crosslinking during MW treatment. MW and WB cause different response behaviors of myofibrillar protein, thereby affecting the catalytic effect of TGase.

引用

页码：45 / 52

页数：8

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