A simple colorimetric method for determination of hydrogen peroxide in plant tissues

被引：149

作者：

Zhou, Biyan

Wang, Jihua

Guo, Zhenfei ^{[1
]}

Tan, Huoquan

Zhu, Xiaochuan

机构：

[1] S China Agr Univ, Coll Life Sci, Guangzhou 510642, Peoples R China

[2] S China Agr Univ, Physiol Lab S China Fruits, Coll Hort, Guangzhou 510642, Peoples R China

来源：

PLANT GROWTH REGULATION | 2006年 / 49卷 / 2-3期

关键词：

4-aminoantipyrine; hydrogen peroxide; peroxidase; plant tissue;

D O I：

10.1007/s10725-006-9000-2

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A simple colorimetric method for determination of hydrogen peroxide in plant materials is described. The method is based on hydrogen peroxide producing a stable red product in reaction with 4-aminoantipyrine and phenol in the presence of peroxidase. Plant tissues was ground with trichloroacetic acid (5% w/v) and extracts were adjusted to pH 8.4 with ammonia solution. Activated charcoal was added to the homogenate to remove pigments, antioxidants and other interfering substances. The colorimetric reagent (pH 5.6) consisted of 4-aminoantipyrine, phenol, and peroxidase. With this method, we have determined the hydrogen peroxide concentration in leaves of eight species which ranged from 0.2 to 0.8 mu mol g(-1) FW. Changes in hydrogen peroxide concentration of Stylosanthes guianensis in response to heat stress are also analyzed using this method.

引用

页码：113 / 118

页数：6

共 25 条

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