A novel mitochondria-targetted near-infrared fluorescent probe for selective and colorimetric detection of sulfite and its application in vitro and vivo

被引：70
作者：
Zeng, Rui-Feng [1 ]
Lan, Jin-Shuai [1 ,2 ]
Wu, Tong [1 ]
Liu, Li [1 ]
Liu, Yun [2 ]
Ho, Rodney J. Y. [3 ]
Ding, Yue [1 ,2 ]
Zhang, Tong [1 ,2 ]
机构：
[1] Shanghai Univ Tradit Chinese Med, Sch Pharm, Shanghai, Peoples R China
[2] Shanghai Univ Tradit Chinese Med, Expt Ctr Teaching & Learning, Shanghai 201203, Peoples R China
[3] Univ Washington, Dept Pharmaceut, Seattle, WA 98195 USA
来源：
FOOD CHEMISTRY | 2020年 / 318卷
基金：
中国国家自然科学基金;
关键词：
SO2; derivatives; Near-infrared fluorescent probe; Mitochondria-target; Fast-testing; Bioimaging; TURN-ON PROBE; SULFUR-DIOXIDE; SO2; DERIVATIVES; RATIOMETRIC DETECTION; LIVING CELLS; BISULFITE; ANIONS;
D O I：
10.1016/j.foodchem.2020.126358
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Overdoses of SO2 and its derivatives (SO32-/HSO3-) in food or organisms are harmful to health. To detect SO32-/HSO3-, a novel NIR fluorescent probe 1, based upon the intramolecular charge transfer (ICT) mechanism, was developed. This probe was easily synthesized, and gave noticeable colorimetric and linear fluorescence changes at 690 nm after reaction with sulfite from 3.13 to 200 mu M. Moreover, probe 1 displayed high sensitivity (LOD = 0.46 mu M), excellent selectivity (among 13 kinds of anions and 3 kinds of biothiols) and quick response (within 30 min) towards SO32-/HSO3-. The SO32-/HSO3- sensing mechanism was confirmed as the Michael addition reaction. Furthermore, the probe showed wide applications for measuring SO32-/HSO3- in real samples, including sugar, tap water, wine and traditional Chinese medicine. The probe could also be used to detect SO32-/HSO3- in mitochondria of HepG2 cells and zebrafish, which suggested potential application for monitoring SO2 derivatives in clinical diagnostics.
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页数：8
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