Amphiphilic Graphene Quantum Dots as Self-Targeted Fluorescence Probes for Cell Nucleus Imaging

被引:54
作者
Wang, Liang [1 ]
Wu, Bin [2 ]
Li, Weitao [1 ]
Wang, Shilong [2 ]
Li, Zhen [3 ]
Li, Ming [1 ]
Pan, Dengyu [4 ]
Wu, Minghong [3 ]
机构
[1] Shanghai Univ, Sch Environm & Chem Engn, Inst Nanochem & Nanobiol, Shanghai 200444, Peoples R China
[2] Tongji Univ, Sch Life Sci & Technol, Res Ctr Translat Med, East Hosp, Shanghai 200092, Peoples R China
[3] Shanghai Univ, Shanghai Inst Appl Radiat, Shanghai 200444, Peoples R China
[4] Shanghai Univ, Sch Environm & Chem Engn, Dept Chem Engn, Shanghai 200444, Peoples R China
基金
中国国家自然科学基金;
关键词
amphiphilic; cell nucleus imaging; graphene quantum dots; self-targeted; POLYHEDRAL OLIGOMERIC SILSESQUIOXANE; CARBON DOTS; 2-PHOTON FLUORESCENCE; GOLD NANOCLUSTERS; NANOPARTICLES; DELIVERY; PEPTIDE; OXIDE; LOCALIZATION; STRATEGY;
D O I
10.1002/adbi.201700191
中图分类号
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号
0805 ; 080501 ; 080502 ;
摘要
Herein, a one-step solvothermal strategy to synthesize amphiphilic, highly fluorescent graphene quantum dots (GQDs) as nucleus-targeted fluorescence probes is reported. The edge-functionalization of GQDs with N and CI ligands renders them amphiphilic and positive charged for boosting membrane crossing and histone binding in the nuclei. The fluorescent properties of the GQDs can be markedly enhanced by CI doping, and thus their fluorescence quantum yield is raised to 30% under optimized CI doping. The self-targeted CQDs exhibit multicolor cell imaging capability for visualization of fine architectures of the nucleus such as the nuclear envelope and nucleoli, which can be associated with their distinct surface features like amphiphilicity and high positive charge. These findings serve as a simple nuclear targeting strategy for cancer diagnosis and therapy.
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页数:8
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