Nucleic acid-mimicking coordination polymer for label-free fluorescent activity assay of histone acetyltransferases

被引：28

作者：

Chen, Siyu ^{[1
]}

Li, Yong ^{[1
]}

Hu, Yufang ^{[1
]}

Han, Yitao ^{[1
]}

Huang, Yan ^{[1
]}

Nie, Zhou ^{[1
]}

Yao, Shouzhuo ^{[1
]}

机构：

[1] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemobiosensing & Chemometr, Changsha 410082, Hunan, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2015年 / 51卷 / 21期

基金：

中国国家自然科学基金;

关键词：

COMPLEXES; CLUSTERS;

D O I：

10.1039/c5cc00067j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A novel and label-free fluorescence assay for histone acetyltransferase (HAT) activity was established via in situ generation of a nucleic acid-mimicking CoA-Au(I) coordination polymer (CP). Moreover, the potency of this assay for HAT-targeted drug discovery was proved by screening HAT inhibitors.

引用

收藏

页码：4469 / 4472

页数：4

相关论文

共 25 条

[1]

Ali- Si- Ali S., 1998, NUCLEIC ACIDS RES, V26, P3869

[2] Au•••Au interactions:: Z′>1 behavior and structural analysis [J].

Anderson, Kirsty M. ;

Goeta, Andres E. ;

Steed, Jonathan W. .

INORGANIC CHEMISTRY, 2007, 46 (16) :6444-6451

[3] STRUCTURE OF COENZYME-A [J].

BADDILEY, J ;

THAIN, EM ;

NOVELLI, GD ;

LIPMANN, F .

NATURE, 1953, 171 (4341) :76-76

[4] Gold nanoparticle size controlled by polymeric Au(I) thiolate precursor size [J].

Brinas, Raymond P. ;

Hu, Minghui ;

Qian, Luping ;

Lymar, Elena S. ;

Hainfeld, James F. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2008, 130 (03) :975-982

[5] Preparation of Protein-like Silver-Cysteine Hybrid Nanowires and Application in Ultrasensitive Immunoassay of Cancer Biomarker [J].

Chen, Wenjuan ;

Zheng, Liyan ;

Wang, Meilan ;

Chi, Yuwu ;

Chen, Guonan .

ANALYTICAL CHEMISTRY, 2013, 85 (20) :9655-9663

[6] Histone acetyl transferases as emerging drug targets [J].

Dekker, Frank J. ;

Haisma, Hidde J. .

DRUG DISCOVERY TODAY, 2009, 14 (19-20) :942-948

[7] Comparative studies of thiol-sensitive fluorogenic probes for HAT assays [J].

Gao, Tielong ;

Yang, Chao ;

Zheng, Yujun George .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2013, 405 (04) :1361-1371

[8] Quantum-Dot-Based FRET Detection of Histone Acetyltransferase Activity [J].

Ghadiali, James E. ;

Lowe, Stuart B. ;

Stevens, Molly M. .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2011, 50 (15) :3417-3420

[9] A continuous, nonradioactive assay for histone acetyltransferases [J].

Kim, Y ;

Tanner, KG ;

Denu, JM .

ANALYTICAL BIOCHEMISTRY, 2000, 280 (02) :308-314

[10] Non-isotopic in vitro assay for histone acetylation [J].

Kuninger, David ;

Lundblad, James ;

Semirale, Anthony ;

Rotwein, Peter .

JOURNAL OF BIOTECHNOLOGY, 2007, 131 (03) :253-260