Nucleic acid-mimicking coordination polymer for label-free fluorescent activity assay of histone acetyltransferases

被引：28
作者：
Chen, Siyu [1 ]
Li, Yong [1 ]
Hu, Yufang [1 ]
Han, Yitao [1 ]
Huang, Yan [1 ]
Nie, Zhou [1 ]
Yao, Shouzhuo [1 ]
机构：
[1] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemobiosensing & Chemometr, Changsha 410082, Hunan, Peoples R China
来源：
CHEMICAL COMMUNICATIONS | 2015年 / 51卷 / 21期
基金：
中国国家自然科学基金;
关键词：
COMPLEXES; CLUSTERS;
D O I：
10.1039/c5cc00067j
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A novel and label-free fluorescence assay for histone acetyltransferase (HAT) activity was established via in situ generation of a nucleic acid-mimicking CoA-Au(I) coordination polymer (CP). Moreover, the potency of this assay for HAT-targeted drug discovery was proved by screening HAT inhibitors.
引用
收藏
页码：4469 / 4472
页数：4
相关论文
共 25 条
  • [1] Ali- Si- Ali S., 1998, NUCLEIC ACIDS RES, V26, P3869
  • [2] Au•••Au interactions:: Z′>1 behavior and structural analysis
    Anderson, Kirsty M.
    Goeta, Andres E.
    Steed, Jonathan W.
    [J]. INORGANIC CHEMISTRY, 2007, 46 (16) : 6444 - 6451
  • [3] STRUCTURE OF COENZYME-A
    BADDILEY, J
    THAIN, EM
    NOVELLI, GD
    LIPMANN, F
    [J]. NATURE, 1953, 171 (4341) : 76 - 76
  • [4] Gold nanoparticle size controlled by polymeric Au(I) thiolate precursor size
    Brinas, Raymond P.
    Hu, Minghui
    Qian, Luping
    Lymar, Elena S.
    Hainfeld, James F.
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2008, 130 (03) : 975 - 982
  • [5] Preparation of Protein-like Silver-Cysteine Hybrid Nanowires and Application in Ultrasensitive Immunoassay of Cancer Biomarker
    Chen, Wenjuan
    Zheng, Liyan
    Wang, Meilan
    Chi, Yuwu
    Chen, Guonan
    [J]. ANALYTICAL CHEMISTRY, 2013, 85 (20) : 9655 - 9663
  • [6] Histone acetyl transferases as emerging drug targets
    Dekker, Frank J.
    Haisma, Hidde J.
    [J]. DRUG DISCOVERY TODAY, 2009, 14 (19-20) : 942 - 948
  • [7] Comparative studies of thiol-sensitive fluorogenic probes for HAT assays
    Gao, Tielong
    Yang, Chao
    Zheng, Yujun George
    [J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2013, 405 (04) : 1361 - 1371
  • [8] Quantum-Dot-Based FRET Detection of Histone Acetyltransferase Activity
    Ghadiali, James E.
    Lowe, Stuart B.
    Stevens, Molly M.
    [J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2011, 50 (15) : 3417 - 3420
  • [9] A continuous, nonradioactive assay for histone acetyltransferases
    Kim, Y
    Tanner, KG
    Denu, JM
    [J]. ANALYTICAL BIOCHEMISTRY, 2000, 280 (02) : 308 - 314
  • [10] Non-isotopic in vitro assay for histone acetylation
    Kuninger, David
    Lundblad, James
    Semirale, Anthony
    Rotwein, Peter
    [J]. JOURNAL OF BIOTECHNOLOGY, 2007, 131 (03) : 253 - 260
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