Evaluation of Enzyme Immunoassays and Real-Time PCR for Detecting Shiga Toxin-Producing Escherichia coli in Southern Alberta, Canada

被引：20

作者：

Chui, Linda ^{[1
,2
]}

Patterson-Fortin, Laura ^{[1
]}

Kuo, Julie ^{[1
]}

Li, Vincent ^{[1
]}

Boras, Valerie ^{[3
]}

机构：

[1] Prov Lab Publ Hlth, Edmonton, AB, Canada

[2] Univ Alberta, Edmonton, AB, Canada

[3] Chinook Reg Hosp, Dept Lab Med, Lethbridge, AB, Canada

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2015年 / 53卷 / 03期

关键词：

HEMOLYTIC-UREMIC-SYNDROME; UNITED-STATES; MULTISTATE OUTBREAK; INFECTIONS; O157/H7; TRANSMISSION; CONSUMPTION; WALKERTON; DISEASE; ONTARIO;

D O I：

10.1128/JCM.03288-14

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Two immunoassays (Shiga Toxin Chek and Shiga Toxin Quik Chek) and real-time PCR were used to detect Shiga toxin-producing Escherichia coli. For enriched culture, the sensitivity and specificity of the three methods ranged from 80.0% to 98.2% and 98.0% to 100.0%, respectively. STEC isolates were identified in 2.6% of the 784 samples.

引用

页码：1019 / 1023

页数：5

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