Nanoparticle microinjection and Raman spectroscopy as tools for nanotoxicology studies

被引：37

作者：

Candeloro, Patrizio ^{[1
]}

Tirinato, Luca ^{[1
]}

Malara, Natalia ^{[1
]}

Fregola, Annalisa ^{[1
]}

Casals, Eudald ^{[2
,3
]}

Puntes, Victor ^{[2
,3
]}

Perozziello, Gerardo ^{[1
]}

Gentile, Francesco ^{[1
,4
]}

Coluccio, Maria Laura ^{[1
,4
]}

Das, Gobind ^{[4
]}

Liberale, Carlo ^{[4
]}

De Angelis, Francesco ^{[4
]}

Di Fabrizio, Enzo ^{[1
,4
]}

机构：

[1] Magna Graecia Univ Catanzaro, Expt & Clin Med Dept, BioNEM Lab, I-88100 Catanzaro, Italy

[2] Inst Catala Nanotecnol, Barcelona, Spain

[3] ICREA, Barcelona, Spain

[4] Italian Inst Technol, Nanostruct Dept, I-16163 Genoa, Italy

来源：

ANALYST | 2011年 / 136卷 / 21期

关键词：

IRON-OXIDE NANOPARTICLES; IN-VITRO; GOLD NANOPARTICLES; QUANTUM DOTS; OXIDATIVE STRESS; MAMMALIAN-CELLS; SINGLE CELLS; TOXICITY; CANCER; TISSUE;

D O I：

10.1039/c1an15313g

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Microinjection techniques and Raman spectroscopy have been combined to provide a new methodology to investigate the cytotoxic effects due to the interaction of nanomaterials with cells. In the present work, this novel technique has been used to investigate the effects of Ag and Fe(3)O(4) nanoparticles on Hela cells. The nanoparticles are microinjected inside the cells and these latter ones are probed by means of Raman spectroscopy after a short incubation time, in order to highlight the first and impulsive mechanisms developed by the cells to counteract the presence of the nanoparticles. The results put in evidence a different behaviour of the cells treated with nanoparticles in comparison with the control cells; these differences are supposed to be generated by an emerging oxidative stress due to the nanoparticles. The achieved results demonstrate the suitability of the proposed method as a new tool for nanotoxicity studies.

引用

页码：4402 / 4408

页数：7

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