Analysis of Pasteurella multocida strains isolated from cattle with haemorrhagic septicaemia by the use of PFGE

被引：0

作者：

Kedrak-Jablonska, Agnieszka ^{[1
]}

Borkowska-Opacka, Bogna ^{[1
]}

机构：

[1] Natl Inst Vet Res, Dept Microbiol, PL-24100 Pulawy, Poland

来源：

BULLETIN OF THE VETERINARY INSTITUTE IN PULAWY | 2007年 / 51卷 / 04期

关键词：

cattle; Pasteurella multocida; haemorrhagic septicaemia; PFGE;

D O I：

暂无

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Forty Pasteurella multocida subsp. multocida strains collected from cattle with haemorrhagic septicaemia belonging to serotype B:2, 5 and two reference strains of serotype B:2, were examined. The strains were isolated in the years 1958 to 1962 and 1985 to 1995. Analysis of the polimorfism of restricted DNA fragments by the use of the pulsed-field gel electrophoresis (PFGE) technique of contour-clamped homogeneous electric field electrophoresis were made. The phylogenetic relationship between the examined strains was calculated by the unweighted pair-group method using arithmetic averages After digestion of chromosomal DNA by using ApaI endonuclease, field isolates from HS cases were homogenous. The presence of an additional restrictive fragment in electrophoretic profile was found only in one strain.

引用

页码：515 / 517

页数：3

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