Lysinibacillus antri sp. nov., isolated from cave soil

A Gram-stain-positive, motile, rod-shaped and endospore-forming strain, SYSU K30002(T), was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-west China. SYSU K30002(T) grew at 28-40 degrees C (optimum, 37 degrees C), at pH 5.0-8.0 (optimum, pH 7.0) and in the presence of 0-4% (w/v) NaCl (optimum in the absence of NaCl). The cell-wall peptidoglycan type was A4 alpha (Lys-Asp). The cell-wall sugars of SYSU K30002(T) were ribose, galactose and mannose, and MK-7 was the menaquinone. The major fatty acids were iso-C-15:0,C-16:1 omega 7c alcohol and iso-C-16:0. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unidentified phospholipids. The G+C content of the genomic DNA was 36.1 mol%. The average nucleotide identity values between SYSU K30002(T) and its closest relatives were below the cut-off level (95-96%) for species delineation. Based on phenotypic, chemotaxonomic and genome comparisons, strain SYSU K30002(T) represents a novel species of the genus Lysinibacillus, for which the name Lysinibacillusantri sp. nov. is proposed. The type strain is SYSU K30002(T) (=KCTC 33955(T)=CGMCC 1.13504(T)).