EVALUATION OF AN SSR MARKER FOR MARKER-ASSISTED SELECTION IN KALE (BRASSICA OLERACEA var. ACEPHALA)

被引:0
作者
Sarikamis, G. [1 ]
Yanmaz, R. [1 ]
Balkaya, A. [2 ]
Belge, B. [1 ]
机构
[1] Ankara Univ, Fac Agr, Dept Hort, TR-06110 Ankara, Turkey
[2] Ondokuz Mayis Univ, Fac Agr, Dept Hort, TR-55139 Samsun, Turkey
来源
BULGARIAN JOURNAL OF AGRICULTURAL SCIENCE | 2010年 / 16卷 / 01期
关键词
Brassica oleracea; SSR; kale; S-TRANSFERASE POLYMORPHISMS; QUANTITATIVE TRAIT LOCI; GLUCOSINOLATE CONTENT; CANCER-RISK; MICROSATELLITES; BROCCOLI; IDENTIFICATION; ACCUMULATION; GENOTYPES; REGION;
D O I
暂无
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
SARIKAMIS, G., R. YANMAZ, A. BALKAYA and B. BELGE, 2010. Evaluation of an SSR marker for marker-assisted selection in kale (Brassica oleracea var. acephala). Bulg. J. Agric. Sci., 16: 36-41 The objective of the current study is to test an SSR marker (Ol12FO2) associated with methylsulphinylalkyl glucosinolates and its potential application for marker assisted selection (MAS) in kale (Brassica oleracea var. acephala). Methylsulphinylalkyl glucosinolates such as glucoiberin and glucoraphanin are known to have health promoting properties and to protect against various forms of cancer. Markers associated with such important traits are important to expedite breeding programs aimed to select for desired traits for further use in generating improved novel lines. The findings of the present study revealed that successful amplifications with genomic DNA from kale genotypes were observed with the SSR marker Ol12FO2 and bands of expected sizes were obtained. It was determined that Ol12FO2 was polymorphic among kale genotypes. The results obtained in the present study demonstrated successful amplification of markers within different vegetable brassicas.
引用
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页码:36 / 41
页数:6
相关论文
共 29 条
[1]  
Balkaya A., 2005, Journal of Vegetable Science, V11, P81, DOI 10.1300/J484v11n04_08
[2]   Morphological characterisation of white head cabbage (Brassica oleracea var. capitata subvar. alba) genotypes in Turkey [J].
Balkaya, A ;
Yanmaz, R ;
Apaydin, A ;
Kar, H .
NEW ZEALAND JOURNAL OF CROP AND HORTICULTURAL SCIENCE, 2005, 33 (04) :333-341
[3]   Promising kale (Brassica oleracea var. acephala) populations from Black Sea region, Turkey [J].
Balkaya, A ;
Yanmaz, R .
NEW ZEALAND JOURNAL OF CROP AND HORTICULTURAL SCIENCE, 2005, 33 (01) :1-7
[4]  
Balkaya A., 2004, CHARACTERIZATION KAL
[5]  
Balkaya A., 2002, TBRK KOOP EKIN, V5, P18
[6]   Marker-assisted increase of genetic diversity in a double-low seed quality winter oilseed rape genetic background [J].
Basunanda, P. ;
Spiller, T. H. ;
Hasan, M. ;
Gehringer, A. ;
Schondelmaier, J. ;
Luehs, W. ;
Friedt, W. ;
Snowdon, R. J. .
PLANT BREEDING, 2007, 126 (06) :581-587
[7]  
Fowke JH, 2003, CANCER RES, V63, P3980
[8]   High-density Brassica oleracea linkage map:: identification of useful new linkages [J].
Gao, Muqiang ;
Li, Genyi ;
Yang, Bo ;
Qiu, Dan ;
Farnham, Mark ;
Quiros, Carlos .
THEORETICAL AND APPLIED GENETICS, 2007, 115 (02) :277-287
[9]   Cruciferous vegetables, mushrooms, and gastrointestinal cancer risks in a multicenter, hospital-based case-control study in Japan [J].
Hara, M ;
Hanaoka, T ;
Kobayashi, M ;
Otani, T ;
Adachi, HY ;
Montani, A ;
Natsukawa, S ;
Shaura, K ;
Koizumi, Y ;
Kasuga, Y ;
Matsuzawa, T ;
Ikekawa, T ;
Sasaki, S ;
Tsugane, S .
NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL, 2003, 46 (02) :138-147
[10]   Association of gene-linked SSR markers to seed glucosinolate content in oilseed rape (Brassica napus ssp. napus) [J].
Hasan, M. ;
Friedt, W. ;
Pons-Kuehnemann, J. ;
Freitag, N. M. ;
Link, K. ;
Snowdon, R. J. .
THEORETICAL AND APPLIED GENETICS, 2008, 116 (08) :1035-1049