2D luminescence imaging of physiological wound oxygenation

被引：50
作者：
Schreml, Stephan [1 ]
Meier, Robert J. [2 ]
Wolfbeis, Otto S. [2 ]
Maisch, Tim [1 ]
Szeimies, Rolf-Markus [3 ]
Landthaler, Michael [1 ]
Regensburger, Johannes [1 ]
Santarelli, Francesco [1 ]
Klimant, Ingo [4 ]
Babilas, Philipp [1 ]
机构：
[1] Univ Hosp Regensburg, Dept Dermatol, D-93053 Regensburg, Germany
[2] Univ Regensburg, Inst Analyt Chem Chemo & Biosensors, Regensburg, Germany
[3] Clin Skin Allergy Phlebol & Environm Med, Recklinghausen, Germany
[4] Graz Univ Technol, Inst Analyt Chem & Food Chem, A-8010 Graz, Austria
来源：
EXPERIMENTAL DERMATOLOGY | 2011年 / 20卷 / 07期
关键词：
epidermal barrier; luminescence imaging; oxygen; split-skin; wound healing; IN-VIVO; EPIDERMAL THICKNESS; SUPERFICIAL WOUNDS; STRATUM-CORNEUM; SKIN; TENSION; SENSORS; SPECTROSCOPY; ATMOSPHERE; DRESSINGS;
D O I：
10.1111/j.1600-0625.2011.01263.x
中图分类号：
R75 [皮肤病学与性病学];
学科分类号：
100206 ;
摘要：
In cutaneous wound healing, the role of oxygen in vivo is poorly understood. We studied wound surface pO(2) during physiological wound healing in humans. Split-thickness skin graft donor sites (n = 12) served as standardized wound models. Wound surface pO(2) was measured at 1, 6 and 14 days after split-skin harvesting using two-dimensional luminescence lifetime imaging (2D-LLI) of palladium(II)-meso-tetraphenyl-tetrabenzoporphyrin (Pd-TPTBP) in polystyrene-co-acrylonitrile (PSAN) particles on transparent foils. In another experiment, we removed the stratum corneum (SC) on the volar forearm (n = 10) by tape strippings to study the impact of the SC on the epidermal oxygen barrier. Split-skin donor site pO(2) significantly decreased during the time course of physiological healing. Regional differences in pO(2) within donor site wounds were visualized for the first time in literature. No difference was found in pO(2) before and after SC removal, showing that the SC is not a major constituent of the epidermal oxygen barrier.
引用
收藏
页码：550 / 554
页数：5
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