The sarcoplasmic reticulum Ca2+ pump:: Inhibition by thapsigargin and enhancement by adenovirus-mediated gene transfer

被引:15
作者
Inesi, G [1 ]
Wade, R [1 ]
Rogers, T [1 ]
机构
[1] Univ Maryland, Sch Med, Dept Biochem & Mol Biol, Baltimore, MD 21201 USA
来源
CARDIAC SARCOPLASMIC RETICULUM FUNCTION AND REGULATION OF CONTRACTILITY | 1998年 / 853卷
关键词
D O I
10.1111/j.1749-6632.1998.tb08267.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The role of the sarcoplasmic reticulum Ca2+ pump in the excitation-contraction coupling of cardiac muscle fibers was evaluated in experiments on SR ATPase inhibition with thapsigargin or, alternatively, on Ca2+ pump enhancement by SR ATPase transgenic expression, We found that thapsigargin, a highly specific and potent inhibitor of the SR ATPase, produces a strong reduction of cytosolic Ca2+ transient and contractile activation in neonatal rat myocytes, in the absence of any other functional effect. On the other hand, Ca2+ pump enhancement by ATPase transgenic expression affects dramatically Ca2+ transient and twitches, resulting in shorter duration and more rapid decay rates. Of particular interest is gene transfer mediated by recombinant adenovirus vectors under control of a cell-specific promoter, resulting in transgenic expression of all myocytes in culture, and no expression in fibroblasts.
引用
收藏
页码:195 / 206
页数:12
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