Histone H3k9 and H3k27 Acetylation Regulates IL-4/STAT6-Mediated Igε Transcription in B Lymphocytes

被引：11

作者：

Fu, Xiao ^{[1
,2
,3
]}

Wang, Xinting ^{[1
,2
,3
]}

Duan, Zhongchao ^{[1
,2
,3
]}

Zhang, Chunyan ^{[1
,2
,3
]}

Fu, Xue ^{[1
,2
,3
]}

Yang, Jie ^{[1
,2
,3
,4
,5
,6
]}

Liu, Xin ^{[1
,2
,3
]}

He, Jinyan ^{[2
,3
,7
]}

机构：

[1] Tianjin Med Univ, Dept Biochem & Mol Biol, Sch Basic Med Sci, Tianjin 300070, CN, Peoples R China

[2] Tianjin Med Univ, Tianjin Key Lab Cellular & Mol Immunol, Tianjin 300070, CN, Peoples R China

[3] Tianjin Med Univ, Key Lab, Educ Minist China, Tianjin 300070, CN, Peoples R China

[4] Tianjin Med Univ, Dept Immunol, Sch Basic Med Sci, Tianjin 300070, CN, Peoples R China

[5] Tianjin Med Univ, Lab Mol Immunol, Res Ctr Basic Med Sci, Tianjin 300070, CN, Peoples R China

[6] Univ Manitoba, Dept Immunol, Winnipeg, MB R3E 0T5, Canada

[7] Tianjin Med Univ, Dept Physiol, Sch Basic Med Sci, Tianjin 300070, CN, Peoples R China

来源：

ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY | 2015年 / 298卷 / 08期

基金：

美国国家科学基金会;

关键词：

IL-4; STAT6; histone modification; Ig epsilon; SOLITARY FIBROUS TUMOR; STAT6; ASTHMA; EXPRESSION; CELLS; IL-4; INFLAMMATION; COACTIVATOR; ACTIVATION; PHENOTYPES;

D O I：

10.1002/ar.23172

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

IL-4 activates STAT6 and causes the subsequent up-regulation of Ig heavy chain germline Ig epsilon via chromatin remodeling involved in B lymphocytes development. STAT6 acts as a molecular switch to regulate the higher-order chromatin remodeling via dynamically orchestrating co-activators (CBP/Tudor-SN) and co-repressors (HDAC1/PSF). Here, we demonstrated that STAT6/Tudor-SN/PSF form a complex, balancing the acetylation and deacetylation states to co-regulate IL-4/STAT6 gene transcription. In addition, we confirmed that IL-4 treatment increased the HATs activity in Ramos cells. As active markers, the expression of H3K9ac and H3K27ac increased after treatment with IL-4. However, transcriptional repressors such as H3K9me3 and H3K27me3 decreased in response to IL-4 stimulation. Moreover, IL-4 treatment enhanced H3 acetylation at the Ig epsilon promoter regions. Our results revealed that the Ig epsilon gene transcription is regulated by histone modifications in the IL-4/STAT6 pathway. The study will provide novel insights into the pathogenesis of allergic diseases. Anat Rec, 298:1431-1439, 2015. (c) 2015 Wiley Periodicals, Inc.

引用

页码：1431 / 1439

页数：9