Histone H3k9 and H3k27 Acetylation Regulates IL-4/STAT6-Mediated Igε Transcription in B Lymphocytes

被引:10
作者
Fu, Xiao [1 ,2 ,3 ]
Wang, Xinting [1 ,2 ,3 ]
Duan, Zhongchao [1 ,2 ,3 ]
Zhang, Chunyan [1 ,2 ,3 ]
Fu, Xue [1 ,2 ,3 ]
Yang, Jie [1 ,2 ,3 ,4 ,5 ,6 ]
Liu, Xin [1 ,2 ,3 ]
He, Jinyan [2 ,3 ,7 ]
机构
[1] Tianjin Med Univ, Dept Biochem & Mol Biol, Sch Basic Med Sci, Tianjin 300070, CN, Peoples R China
[2] Tianjin Med Univ, Tianjin Key Lab Cellular & Mol Immunol, Tianjin 300070, CN, Peoples R China
[3] Tianjin Med Univ, Key Lab, Educ Minist China, Tianjin 300070, CN, Peoples R China
[4] Tianjin Med Univ, Dept Immunol, Sch Basic Med Sci, Tianjin 300070, CN, Peoples R China
[5] Tianjin Med Univ, Lab Mol Immunol, Res Ctr Basic Med Sci, Tianjin 300070, CN, Peoples R China
[6] Univ Manitoba, Dept Immunol, Winnipeg, MB R3E 0T5, Canada
[7] Tianjin Med Univ, Dept Physiol, Sch Basic Med Sci, Tianjin 300070, CN, Peoples R China
来源
ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY | 2015年 / 298卷 / 08期
基金
美国国家科学基金会;
关键词
IL-4; STAT6; histone modification; Ig epsilon; SOLITARY FIBROUS TUMOR; STAT6; ASTHMA; EXPRESSION; CELLS; IL-4; INFLAMMATION; COACTIVATOR; ACTIVATION; PHENOTYPES;
D O I
10.1002/ar.23172
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
IL-4 activates STAT6 and causes the subsequent up-regulation of Ig heavy chain germline Ig epsilon via chromatin remodeling involved in B lymphocytes development. STAT6 acts as a molecular switch to regulate the higher-order chromatin remodeling via dynamically orchestrating co-activators (CBP/Tudor-SN) and co-repressors (HDAC1/PSF). Here, we demonstrated that STAT6/Tudor-SN/PSF form a complex, balancing the acetylation and deacetylation states to co-regulate IL-4/STAT6 gene transcription. In addition, we confirmed that IL-4 treatment increased the HATs activity in Ramos cells. As active markers, the expression of H3K9ac and H3K27ac increased after treatment with IL-4. However, transcriptional repressors such as H3K9me3 and H3K27me3 decreased in response to IL-4 stimulation. Moreover, IL-4 treatment enhanced H3 acetylation at the Ig epsilon promoter regions. Our results revealed that the Ig epsilon gene transcription is regulated by histone modifications in the IL-4/STAT6 pathway. The study will provide novel insights into the pathogenesis of allergic diseases. Anat Rec, 298:1431-1439, 2015. (c) 2015 Wiley Periodicals, Inc.
引用
收藏
页码:1431 / 1439
页数:9
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