Evaluation of a High-Throughput Diagnostic System for Detection of HIV-1 in Dried Blood Spot Samples from Infants in Mozambique

被引：10

作者：

Jani, Ilesh V. ^{[2
]}

Sabatier, Jennifer ^{[1
]}

Vubil, Adolfo ^{[2
]}

Subbarao, Shambavi ^{[1
]}

Bila, Dulce ^{[2
]}

de Sousa, Amina ^{[2
]}

Mabunda, Nedio ^{[2
]}

Garcia, Albert ^{[1
]}

Skaggs, Beth ^{[3
]}

Ellenberger, Dennis ^{[1
]}

Ramos, Artur ^{[1
]}

机构：

[1] Ctr Dis Control & Prevent, Atlanta, GA USA

[2] Inst Nacl Saude, Maputo, Mozambique

[3] Ctr Dis Control & Prevent, Maputo, Mozambique

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2012年 / 50卷 / 04期

关键词：

IMMUNODEFICIENCY-VIRUS HIV; ANTIRETROVIRAL-THERAPY; PERFORMANCE; ASSAY; MORTALITY; INFECTION; AGREEMENT; CHILDREN; AFRICA; TESTS;

D O I：

10.1128/JCM.00107-12

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We performed a comparative analysis between Roche Amplicor HIV-1 DNA test and CAPTAQ assay for the detection of HIV in 830 dried blood spot (DBS) pediatric samples collected in Mozambique. Our results demonstrated no statistical difference between these assays. The CAPTAQ assay approached nearly 100% repeatability/accuracy. The increased throughput of testing with minimal operator interference in performing the CAPTAQ assay clearly demonstrated that this method is an improvement over the Roche Amplicor HIV-1 DNA test, version 1.5.

引用

收藏

页码：1458 / 1460

页数：3

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