Neuroprotective and anti-inflammatory effects of flavonoids isolated from Rhus verniciflua in neuronal HT22 and microglial BV2 cell lines

被引:141
作者
Cho, Namki
Choi, Ji Hoon
Yang, Heejung
Jeong, Eun Ju
Lee, Ki Yong
Kim, Young Choong
Sung, Sang Hyun [1 ]
机构
[1] Seoul Natl Univ, Coll Pharm, Seoul 151742, South Korea
基金
新加坡国家研究基金会;
关键词
Rhus verniciflua Stokes; Neuroprotective; Anti-inflammatory; Flavonoids; OXIDATIVE STRESS; CYTOTOXICITY; ANTIOXIDANT; GLUTATHIONE; GLUTAMATE;
D O I
10.1016/j.fct.2012.03.052
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
The neuroprotective and anti-inflammatory activities of the methanolic extract of Rhus verniciflua Stokes (Anacardiaceae) were investigated with mouse hippocampal and microglial cells. Bioactivity-guided isolation yielded 10 flavonoids including fustin (1), fisetin (2), sulfuretin (3), butein (4), butin (5), eriodictyol (6), morin hydrate (7), quercetin (8), kaempferol (9) and isoliquiritigenin (10). Among the isolated flavonoids, compounds 2-5 significantly protected the murine hippocampal HT22 cells against glutamate-induced neurotoxicity and attenuated reactive oxygen species (ROS) generations. In addition, these flavonoids significantly maintained antioxidative defense systems preserving the activities of superoxide dismutase (SOD), glutathione reductase (GR), glutathione peroxidase (GSH-Px) and the content of glutathione (GSH) decreased by glutamate insult. These compounds also showed significant inhibitory effects on LPS-induced nitric oxide (NO) production in BV2 cells. Especially, compound 4 dose-dependently suppressed the expression of both inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). These results suggest that these flavonoids possess therapeutic potentials as a multipotent agent against neurodegenerative diseases related to oxidative stress and pathological inflammatory responses. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1940 / 1945
页数:6
相关论文
共 29 条
[1]  
CARLBERG I, 1975, J BIOL CHEM, V250, P5475
[2]   Nitric oxide synthases:: targets for therapeutic strategies in neurological diseases [J].
Chabrier, PE ;
Demerlé-Pallardy, C ;
Auguet, M .
CELLULAR AND MOLECULAR LIFE SCIENCES, 1999, 55 (8-9) :1029-1035
[3]   Efficacy and Safety of Rhus verniciflua Stokes Extracts in Patients with Previously Treated Advanced Non-Small Cell Lung Cancer [J].
Cheon, Seong Ha ;
Kim, Kyung Seok ;
Kim, Sehyun ;
Jung, Hyun Sik ;
Choi, Won Cheol ;
Eo, Wan Kyu .
FORSCHENDE KOMPLEMENTARMEDIZIN, 2011, 18 (02) :77-83
[4]   OXIDATIVE STRESS, GLUTAMATE, AND NEURODEGENERATIVE DISORDERS [J].
COYLE, JT ;
PUTTFARCKEN, P .
SCIENCE, 1993, 262 (5134) :689-695
[5]   PROTEIN-KINASE-C ACTIVATION INHIBITS GLUTAMATE-INDUCED CYTOTOXICITY IN A NEURONAL CELL-LINE [J].
DAVIS, JB ;
MAHER, P .
BRAIN RESEARCH, 1994, 652 (01) :169-173
[6]  
FLOHE L, 1984, METHOD ENZYMOL, V105, P114
[7]   Abnormalities in oxidative processes in non-neuronal tissues from patients with Alzheimer's disease [J].
Gibson, Gary E. ;
Zhang, Hui .
JOURNAL OF ALZHEIMERS DISEASE, 2001, 3 (03) :329-338
[8]   SECRETED FORMS OF BETA-AMYLOID PRECURSOR PROTEIN PROTECT HIPPOCAMPAL-NEURONS AGAINST AMYLOID BETA-PEPTIDE-INDUCED OXIDATIVE INJURY [J].
GOODMAN, Y ;
MATTSON, MP .
EXPERIMENTAL NEUROLOGY, 1994, 128 (01) :1-12
[9]   Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships [J].
Heim, KE ;
Tagliaferro, AR ;
Bobilya, DJ .
JOURNAL OF NUTRITIONAL BIOCHEMISTRY, 2002, 13 (10) :572-584
[10]   Cytotoxicity of urushiols isolated from sap of Korean lacquer tree (Rhus vernicifera stokes) [J].
Hong, DH ;
Han, SB ;
Lee, CW ;
Park, SH ;
Jeon, YJ ;
Kim, MJ ;
Kwak, SS ;
Kim, KM .
ARCHIVES OF PHARMACAL RESEARCH, 1999, 22 (06) :638-641