Increasing glutathione formation by functional expression of the γ-glutamylcysteine synthetase gene in Saccharomyces cerevisiae

被引：32

作者：

Fan, XY

He, XP

Guo, XN

Qu, N

Wang, CL

Zhang, BR ^{[1
]}

机构：

[1] Acad Sinica, Inst Microbiol, Ctr Biotechnol, Beijing 100080, Peoples R China

[2] Tianjin Univ Sci & Technol, Dept Biotechnol, Tianjin 300222, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2004年 / 26卷 / 05期

关键词：

expression; gamma-glutamylcysteine synthetase; glutathione; Saccharomyces cerevisiae;

D O I：

10.1023/B:BILE.0000018261.14427.e8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A recombinant plasmid, pGMF, containing a gamma-glutamylcysteine synthetase gene (GSH-I) from Saccharomyces cerevisiae, was constructed with a copper-resistance gene as the selection marker and was introduced into S. cerevisiae YSF-31. The glutathione content of the recombinant strain was 1.5-fold (13.1 mg g dry cells(-1)) of that in the host strain.

引用

页码：415 / 417

页数：3

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