Stable attachment of the HMB-linker to continuous cellulose membranes for parallel solid phase spot synthesis

Peptides and other oligomeric molecules were prepared on continuous cellulose membranes using a stably attached p-hydroxymethyl-benzoic acid (HMB)(1) linker. After attachment of N-protected 1,2-epoxy-propylamine to the support via an ether bond, the HMB linker was coupled to this construct resulting in a orthogonal safety-catch linkage suitable for solid phase synthesis. As an application, several peptides, peptoids and carbohydrate-peptide conjugates were prepared and analyzed by HPLC and mass spectrometry. (C) 1997, Elsevier Science Ltd.