Involvement of actin cytoskeleton in macrophage apoptosis induced by cationic liposomes

被引：11

作者：

Takano, Katsuki ^{[1
]}

Sato, Kaori ^{[1
]}

Negishi, Yoichi ^{[1
]}

Aramaki, Yukihiko ^{[1
]}

机构：

[1] Tokyo Univ Pharm & Life Sci, Hachioji, Tokyo 1920392, Japan

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 2012年 / 518卷 / 01期

关键词：

Apoptosis; Cationic liposomes; Actin; PKC delta; Reactive oxygen species; KINASE C-DELTA; RAW264.7; CELLS; GENE-THERAPY; PROTEOGLYCANS; INDUCTION; TOXICITY; VECTORS; PATHWAY;

D O I：

10.1016/j.abb.2011.12.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We clarified whether actin cytoskeleton is involved in the macrophage apoptosis induced by cationic liposomes composed of stearylamine (SA-liposomes). Externalization of phosphatidylserine induced by SA-liposomes was suppressed by cytochalasin D, a specific inhibitor of polymerization of F-actin. Furthermore, activation of PKCS and reactive oxygen species (ROS) generation, which could be involved in the macrophage apoptosis, were inhibited by cytochalasin D. Microscopical observation revealed the co-localization of 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (Dil)-labeled SA-liposomes and fluorescein-labeled phalloidin, which specifically binds to F-actin, and this co-localization was also inhibited by cytochalasin D. Co-localization of SA-liposomes and F-actin was also inhibited by the pre-treatment of cells with chondroitinase ABC. These findings could be the first observation concerning the contribution of the proteoglycan-actin cytoskeleton-ROS generation pathway to apoptosis induced by SA-liposomes in macrophages. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：89 / 94

页数：6

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