Salvage and storage of infectious disease protein targets in the SSGCID high-throughput crystallization pathway using microfluidics

被引：1

作者：

Christensen, Jeff ^{[1
]}

Gerdts, Cory J. ^{[2
]}

Clifton, Mathew C. ^{[2
]}

Stewart, Lance ^{[2
]}

机构：

[1] Emerald BioStruct, Bainbridge Isl, WA 98110 USA

[2] Emerald BioSyst, Bainbridge Isl, WA 98110 USA

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2011年 / 67卷

基金：

美国国家卫生研究院;

关键词：

OPTIMIZATION; SYSTEM;

D O I：

10.1107/S1744309111023232

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The MPCS Plug Maker is a microcapillary-based protein-crystallization system for generating diffraction-ready crystals from nanovolumes of protein. Crystallization screening using the Plug Maker was used as a salvage pathway for proteins that failed to crystallize during the initial observation period using the traditional sitting-drop vapor-diffusion method. Furthermore, the CrystalCards used to store the crystallization experiments set up by the Plug Maker are shown be a viable container for long-term storage of protein crystals without a discernable loss of diffraction quality with time. Use of the Plug Maker with SSGCID proteins is demonstrated to be an effective crystal-salvage and storage method.

引用

收藏

页码：1022 / 1026

页数：5

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