Metrological support for DNA sequencing

被引：2

作者：

Golubev, S. S. ^{[1
]}

Kononogov, S. A. ^{[1
]}

Kudeyarov, Yu A. ^{[1
]}

Mardanov, A. V. ^{[2
]}

Nikolaeva, P. Yu ^{[1
]}

Ravin, N. V. ^{[2
]}

Skryabin, K. G. ^{[2
]}

机构：

[1] All Russia Res Inst Metrol Serv VNIIMS, Moscow, Russia

[2] Russian Acad Sci, Ctr Bioengn, Moscow, Russia

来源：

MEASUREMENT TECHNIQUES | 2012年 / 55卷 / 03期

关键词：

DNA molecule; nucleotide sequence; complementarity; pyrophosphate sequencing (pyrosequencing); reference material; plasmid pUC18;

D O I：

10.1007/s11018-012-9962-6

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

We discuss the operating principle of a pyrosequencer, which is an instrument for sequential reading of the nucleotide sequence of DNA molecules in pyrophosphate sequencing (pyrosequencing). We formulate the basic principles of metrological support for DNA sequencing and we present the results of studies of the metrological characteristics of the GS FLX pyrosequencer. We list the major problems encountered in designing a system for metrological support of DNA sequencing.

引用

页码：345 / 350

页数：6

共 4 条

[1]

[Anonymous], 2010, INT VOCABULARY METRO

[2]

Golubev S. S., 2011, IZMER TEKH, P45

[3]

Kuznetsov V. A., 2005, METROLOGY

[4] Genome sequencing in microfabricated high-density picolitre reactors [J].

Margulies, M ;

Egholm, M ;

Altman, WE ;

Attiya, S ;

Bader, JS ;

Bemben, LA ;

Berka, J ;

Braverman, MS ;

Chen, YJ ;

Chen, ZT ;

Dewell, SB ;

Du, L ;

Fierro, JM ;

Gomes, XV ;

Godwin, BC ;

He, W ;

Helgesen, S ;

Ho, CH ;

Irzyk, GP ;

Jando, SC ;

Alenquer, MLI ;

Jarvie, TP ;

Jirage, KB ;

Kim, JB ;

Knight, JR ;

Lanza, JR ;

Leamon, JH ;

Lefkowitz, SM ;

Lei, M ;

Li, J ;

Lohman, KL ;

Lu, H ;

Makhijani, VB ;

McDade, KE ;

McKenna, MP ;

Myers, EW ;

Nickerson, E ;

Nobile, JR ;

Plant, R ;

Puc, BP ;

Ronan, MT ;

Roth, GT ;

Sarkis, GJ ;

Simons, JF ;

Simpson, JW ;

Srinivasan, M ;

Tartaro, KR ;

Tomasz, A ;

Vogt, KA ;

Volkmer, GA .

NATURE, 2005, 437 (7057) :376-380

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