Knock-down of the 37-kDa/67-kDa laminin receptor in mouse brain by transgenic expression of specific antisense LRP RNA

被引：17
作者：
Leucht, C
Vana, K
Renner-Müller, I
Dormont, D
Lasmézas, CI
Wolf, E
Weiss, S
机构：
[1] Univ Munich, Genzentrum Inst Biochem, Mol Biol Lab, D-81377 Munich, Germany
[2] Genzentrum Inst Mol Tierzucht, D-81377 Munich, Germany
[3] CEA, Lab Prion Pathogenesis, Serv Neurovirol DRM DSV, F-92265 Fontenay Aux Roses, France
来源：
TRANSGENIC RESEARCH | 2004年 / 13卷 / 01期
关键词：
antisense RNA; laminin receptor; LRP/LR; neuron-specific enolase (NSE); prion; PrP;
D O I：
10.1023/B:TRAG.0000017177.35197.89
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The 37-kDa/67-kDa laminin receptor (LRP/LR) plays a major role in the propagation of PrPSc, the abnormal form of the prion protein. In order to ablate the expression of LRP/LR in mouse brain we generated transgenic mice ectopically expressing antisense LRP RNA in the brain under control of the neuron-specific enolase (NSE) promoter. Hemizygous transgenic mice TgN(NSEasLRP)2 showed a significant reduction of LRP/LR protein levels in hippocampal and cerebellar brain regions. These mice might act as powerful tools to investigate the role of the laminin receptor in scrapie pathogenesis.
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收藏
页码：81 / 85
页数：5
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