Beneficial effect of silkworm hemolymph on a CHO cell system: Inhibition of apoptosis and increase of EPO production

被引:47
作者
Choi, SS [1 ]
Rhee, WJ [1 ]
Park, TH [1 ]
机构
[1] Seoul Natl Univ, Sch Chem & Biol Engn, Seoul 151744, South Korea
关键词
silkworm hemolymph (SH); apoptosis; Chinese hamster ovary (CHO); erythropoietin (EPO); sodium butyrate;
D O I
10.1002/bit.20550
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
To produce erythropoietin (EPO), Chinese hamster ovary (CHO) cells were first cultured in a medium containing FBS (growth medium) and then in a serumfree medium containing sodium butyrate (production medium). Sodium butyrate increases recombinant protein production, but also induces apoptosis, which reduces cell viability and productivity. In a previous study, we found that silkworm hemolymph (SH), an insect serum, inhibits the apoptosis of insect and mammalian cells. To overcome sodium butyrate-induced apoptosis, we added SH to growth medium. This pretreatment with SH inhibited the sodium butyrate-induced apoptosis of CHO cells and consequently increased their longevity and their ability to produce EPO. As a result, the volumetric productivity of EPO was increased five-fold. SH was found to inhibit cytochrome c release from mitochondria into the cytosol, and prevented the activation of caspase-3 and other subsequent caspase reactions. (c) 2005 Wiley Periodicals, Inc.
引用
收藏
页码:793 / 800
页数:8
相关论文
共 39 条
  • [1] Human ICE/CED-3 protease nomenclature
    Alnemri, ES
    Livingston, DJ
    Nicholson, DW
    Salvesen, G
    Thornberry, NA
    Wong, WW
    Yuan, JY
    [J]. CELL, 1996, 87 (02) : 171 - 171
  • [2] SODIUM BUTYRATE INHIBITS HISTONE DEACETYLATION IN CULTURED-CELLS
    CANDIDO, EPM
    REEVES, R
    DAVIE, JR
    [J]. CELL, 1978, 14 (01) : 105 - 113
  • [3] N-acetylcysteine increases the biosynthesis of recombinant EPO in apoptotic Chinese hamster ovary cells
    Chang, KH
    Kim, KS
    Kim, JH
    [J]. FREE RADICAL RESEARCH, 1999, 30 (02) : 85 - 91
  • [4] Inhibition of human cell apoptosis by silkworm hemolymph
    Choi, SS
    Rhee, WJ
    Park, TH
    [J]. BIOTECHNOLOGY PROGRESS, 2002, 18 (04) : 874 - 878
  • [5] DORNER AJ, 1989, J BIOL CHEM, V264, P20602
  • [6] Efficient production of recombinant protein in Spodoptera frugiperda AcNPV system utilizing silkworm hemolymph
    Ha, SH
    Park, TH
    [J]. BIOTECHNOLOGY LETTERS, 1997, 19 (11) : 1087 - 1091
  • [7] Ha SH, 1996, BIOTECHNOL TECH, V10, P401
  • [8] MOLECULAR-PROPERTIES AND BIOSYNTHESIS OF MAJOR PLASMA-PROTEINS IN BOMBYX-MORI
    IZUMI, S
    FUJIE, J
    YAMADA, S
    TOMINO, S
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1981, 670 (02) : 222 - 229
  • [9] Inhibition of apoptosis by a Bombyx mori gene
    Kim, EJ
    Rhee, WJ
    Park, TH
    [J]. BIOTECHNOLOGY PROGRESS, 2004, 20 (01) : 324 - 329
  • [10] Anti-apoptosis engineering
    Kim, EJ
    Park, TH
    [J]. BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, 2003, 8 (02) : 76 - 82