Identification of CfiA coding genes in Bacteroides fragilis isolates recovered in Argentina. Inconsistencies in CfiA organization and nomenclature

被引：9

作者：

Litterio, Mirta R. ^{[1
]}

Cejas, Daniela ^{[2
,3
]}

Gutkind, Gabriel ^{[2
,3
]}

Radice, Marcela ^{[2
,3
]}

机构：

[1] Hosp Pediat SAMIC Prof Dr Juan P Garrahan, Combate Pozos 1881, Buenos Aires, DF, Argentina

[2] Univ Buenos Aires, Fac Farm & Bioquim, Catedra Microbiol, Junin 956, Buenos Aires, DF, Argentina

[3] Consejo Nacl Invest Cient & Tecn, Buenos Aires, DF, Argentina

来源：

ANAEROBE | 2017年 / 48卷

关键词：

CfiA metallo-beta-lactamase; Bacteroides fragilis; Carbapenem resistance; ANTIBIOTIC-RESISTANCE; ANTIMICROBIAL SUSCEPTIBILITY; EMERGING RESISTANCE; IMIPENEM-RESISTANT; ANAEROBIC-BACTERIA; EXPRESSION; CEFOXITIN; INSERTION; SEQUENCE; REVEALS;

D O I：

10.1016/j.anaerobe.2017.10.003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

CfiA (CcrA) metallo-beta-lactamase is the main carbapenem resistance mechanism in B. fragilis. From cfiA positive isolates detected in a previous surveillance study, 3 displayed resistance to imipenem while the remaining were susceptible. The aim of this study was to identify the cfiA alleles and to analyze the presence of IS elements in their upstream regions. CfiA-1, CfiA-4, CfiA-13, CfiA-19 and CfiA-22 were detected. IS elements belonging to 1521 family and 15942 group were identified upstream to cfiA in the 3 imipenem resistant isolates. We present an exhaustive analysis of cfiA/CfiA registers in databases, illustrating the inconsistencies in both organization and nomenclature. According to this analysis CfiA family comprises nowadays 15 different CfiA variants coded by 24 cfiA sequences. Curation of CfiA database is mandatory, if not new cfiA admission at GenBank will contribute to make this classification more complex. (C) 2017 Elsevier Ltd. All rights reserved.

引用

页码：257 / 261

页数：5

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