Rhodnius prolixus salivary nitrophorins display heme-peroxidase activity

被引：8

作者：

Ribeiro, JMC ^{[1
]}

机构：

[1] NIAID, Parasit Dis Lab, Bethesda, MD 20892 USA

来源：

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY | 1998年 / 28卷 / 12期

关键词：

hematophagy; insect; platelet; hemostasis; saliva; blood-feeding; nitrophorin;

D O I：

10.1016/S0965-1748(98)00096-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rhodnius prolixus is a blood feeding triatomine bug that contains salivary nitric oxide bound to hemoproteins previously named nitrophorins. Nitrophorins, in addition to storing and transporting NO, have two other functions such as anti-histaminic and anticlotting (displayed by nitrophorin 2 only). Additionally, nitrophorins display a thiol oxidase reaction, where cysteine is oxidized to cystine with the production of hydrogen peroxide. In this paper the heme-peroxidase reaction of nitrophorins is described. The heme moiety of nitrophorins is destroyed by addition of cysteine or hydrogen peroxide. No biliverdin is produced during this reaction. We have also found that during the thiol oxidase reaction, nitrophorins can destroy norepinephrine, conferring an additional vasodilatory competence for this class of salivary molecules. Published by Elsevier Science Ltd. All rights reserved.

引用

页码：1051 / 1057

页数：7

共 33 条

[1] Nitric oxide binding and crystallization of recombinant nitrophorin I, a nitric oxide transport protein from the blood-sucking bug Rhodnius prolixus
Andersen, JF
Champagne, DE
Weichsel, A
Ribeiro, JMC
Balfour, CA
Dress, V
Montfort, WR
[J]. BIOCHEMISTRY, 1997, 36 (15) : 4423 - 4428
[2] ANTONINI E, 1971, FRONT BIOL, P1
[3] HEME DEGRADATION IN THE PRESENCE OF GLUTATHIONE - A PROPOSED MECHANISM TO ACCOUNT FOR THE HIGH-LEVELS OF NONHEME IRON FOUND IN THE MEMBRANES OF HEMOGLOBINOPATHIC RED-BLOOD-CELLS
ATAMNA, H
GINSBURG, H
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (42) : 24876 - 24883
[4] BONNETT R, 1975, J CHEM SOC P1, V1, P224
[5] BONNETT R, 1973, P NEW YORK ACAD SCI, V206, P722
[6] The cutaneous microcirculation: Ultrastructure and microanatomical organization
Braverman, IM
[J]. MICROCIRCULATION, 1997, 4 (03) : 329 - 340
[7] THE CONCENTRATIONS OF CYSTEINE AND CYSTINE IN HUMAN BLOOD PLASMA
BRIGHAM, MP
STEIN, WH
MOORE, S
[J]. JOURNAL OF CLINICAL INVESTIGATION, 1960, 39 (11) : 1633 - 1638
[8] LOSS OF HEME AND HEMOPROTEINS DURING THE GENERATION OF SUPEROXIDE ANION AND HYDROGEN-PEROXIDE - A PATHWAY NOT INVOLVING PRODUCTION OF CARBON-MONOXIDE
CANTONI, L
GIBBS, AH
DEMATTEIS, F
[J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY, 1981, 13 (07): : 823 - 830
[9] PURIFICATION, PARTIAL CHARACTERIZATION, AND CLONING OF NITRIC OXIDE-CARRYING HEME-PROTEINS (NITROPHORINS) FROM SALIVARY-GLANDS OF THE BLOODSUCKING INSECT RHODNIUS-PROLIXUS
CHAMPAGNE, DE
NUSSENZVEIG, RH
RIBEIRO, JMC
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (15) : 8691 - 8695
[10] HEME CATABOLISM - A NEW LOOK AT SUBSTRATES AND ENZYMES
FRYDMAN, RB
FRYDMAN, B
[J]. ACCOUNTS OF CHEMICAL RESEARCH, 1987, 20 (07) : 250 - 256

← 1 2 3 4 →