Simultaneous detection of mutations associated with resistance to macrolides and quinolones in Campylobacter jejuni and C-coli using a PCR-line probe assay

被引：28
作者：
Niwa, H
Chuma, T
Okamoto, K
Itoh, K
机构：
[1] Univ Tokyo, Grad Sch Agr & Life Sci, Lab Vet Publ Hlth, Bunkyo Ku, Tokyo 1138657, Japan
[2] Kagoshima Univ, Fac Agr, Dept Vet Med, Lab Vet Publ Hlth, Kagoshima 8900065, Japan
来源：
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS | 2003年 / 22卷 / 04期
关键词：
Campylobacter jejuni; Campylobacter coli; antibiotic resistance; macrolide; quinolone; reverse hybridization;
D O I：
10.1016/S0924-8579(03)00178-X
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Quinolone and macrolide resistance of Campylobacter jejuni and Campylobacter coli mainly depend on a mutation in gyrA and in 23S rDNA, respectively. In order to detect quinolone and/or macrolide resistant C Jejuni and C coli strains, a macrolide and quinolone line probe assay (MQ-LiPA) was developed and 42 C jejuni and C coli strains were tested to evaluate the efficiency of the assay. Profiles of the mutations in 23S rDNA and in gyrA characterized by MQ-LiPA agreed with resistance to macrolides and quinolones. MQ-LiPA is a rapid and simple method for simultaneous detection of quinolone and macrolide resistance of C jejuni and C coli. We could also discriminate between C. jejuni and C. coli using probes for detection of gyrA mutations in MQ-LiPA. (C) 2003 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
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收藏
页码：374 / 379
页数：6
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