A Label-Free Fluorescent DNA Calculator Based on Gold Nanoparticles for Sensitive Detection of ATP

被引：4
|
作者：
Zhang, Jingjing [1 ]
Zhang, Shizhi [1 ]
Niu, Chaoqun [1 ]
Liu, Chen [1 ]
Du, Jie [1 ]
Chen, Yong [1 ]
机构：
[1] Hainan Univ, Inst Trop Agr & Forestry, Coll Mat & Chem Engn,Coll Informat Sci & Technol, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Hainan, Peoples R China
来源：
MOLECULES | 2018年 / 23卷 / 10期
基金：
海南省自然科学基金;
关键词：
ATP detection; label-free fluorescence; enzyme-free; gold nanoparticles; DNA calculator; ADENOSINE 5'-TRIPHOSPHATE; SELECTIVE DETECTION; PROBE; SILVER; IONS; RECOVERY;
D O I：
10.3390/molecules23102494
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Herein we described a deoxyribonucleic acid (DNA) calculator for sensitive detection of the determination of adenosine triphosphate (ATP) using gold nanoparticles (GNP) and PicoGreen fluorescence dye as signal transducer, and ATP and single-stranded DNA (DNA-M') as activators. The calculator-related performances including linearity, reaction time, logic gate, and selectivity were investigated, respectively. The results revealed that this oligonucleotide sensor was highly sensitive and selective. The detection range was 50-500 nmol/L (R-2 = 0.99391) and the detection limit was 46.5 nmol/L. The AND DNA calculator was successfully used for the ATP detection in human urine. Compared with other methods, this DNA calculator has the characteristics of being label-free, non-enzymic, simple, and highly sensitive.
引用
收藏
页数：10
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