A Label-Free Fluorescent DNA Calculator Based on Gold Nanoparticles for Sensitive Detection of ATP

被引：4

作者：

Zhang, Jingjing ^{[1
]}

Zhang, Shizhi ^{[1
]}

Niu, Chaoqun ^{[1
]}

Liu, Chen ^{[1
]}

Du, Jie ^{[1
]}

Chen, Yong ^{[1
]}

机构：

[1] Hainan Univ, Inst Trop Agr & Forestry, Coll Mat & Chem Engn,Coll Informat Sci & Technol, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Hainan, Peoples R China

来源：

MOLECULES | 2018年 / 23卷 / 10期

基金：

海南省自然科学基金;

关键词：

ATP detection; label-free fluorescence; enzyme-free; gold nanoparticles; DNA calculator; ADENOSINE 5'-TRIPHOSPHATE; SELECTIVE DETECTION; PROBE; SILVER; IONS; RECOVERY;

D O I：

10.3390/molecules23102494

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Herein we described a deoxyribonucleic acid (DNA) calculator for sensitive detection of the determination of adenosine triphosphate (ATP) using gold nanoparticles (GNP) and PicoGreen fluorescence dye as signal transducer, and ATP and single-stranded DNA (DNA-M') as activators. The calculator-related performances including linearity, reaction time, logic gate, and selectivity were investigated, respectively. The results revealed that this oligonucleotide sensor was highly sensitive and selective. The detection range was 50-500 nmol/L (R-2 = 0.99391) and the detection limit was 46.5 nmol/L. The AND DNA calculator was successfully used for the ATP detection in human urine. Compared with other methods, this DNA calculator has the characteristics of being label-free, non-enzymic, simple, and highly sensitive.

引用

页数：10

共 50 条

[1] A Label-Free Fluorescent DNA Machine for Sensitive Cyclic Amplification Detection of ATP
Zhang, Jingjing
Han, Jialun
Feng, Shehong
Niu, Chaoqun
Liu, Chen
Du, Jie
Chen, Yong
MATERIALS, 2018, 11 (12)
[2] A Label-Free Fluorescent AND Logic Gate Aptasensor for Sensitive ATP Detection
Zhang, Jingjing
Yang, Chunzheng
Niu, Chaoqun
Liu, Chen
Cai, Xuepin
Du, Jie
Chen, Yong
SENSORS, 2018, 18 (10)
[3] Label-Free Detection of Ag+ Based on Gold Nanoparticles and Ag+-Specific DNA
Pu, Wendan
Zhao, Zhao
Wu, Liping
Liu, Yue
Zhao, Huawen
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, 2015, 15 (08) : 5524 - 5529
[4] A label-free fluorescent sensor for rapid and sensitive detection of ctDNA based on fluorescent PDA nanoparticles
Yang, Xiao
Huang, Yang
Yang, Siyi
Tang, Miao
Liu, Juan
Shen, Jinhui
Fa, Huanbao
Huo, Danqun
Hou, Changjun
Yang, Mei
ANALYST, 2023, 148 (19) : 4885 - 4896
[5] Label-free detection of adenosine based on fluorescence resonance energy transfer between fluorescent silica nanoparticles and unmodified gold nanoparticles
Qiang, Weibing
Liu, Haiping
Li, Wei
Chen, Xiang
Xu, Danke
ANALYTICA CHIMICA ACTA, 2014, 828 : 92 - 98
[6] Highly Sensitive Colorimetric Detection of Ochratoxin A by a Label-Free Aptamer and Gold Nanoparticles
Luan, Yunxia
Chen, Jiayi
Li, Cheng
Xie, Gang
Fu, Hailong
Ma, Zhihong
Lu, Anxiang
TOXINS, 2015, 7 (12): : 5377 - 5385
[7] A sensitive label-free impedimetric DNA biosensor based on silsesquioxane-functionalized gold nanoparticles for Zika Virus detection
Steinmetz, Marines
Lima, Dhesmon
Viana, Adriano Goncalves
Fujiwara, Sergio Toshio
Pessoa, Christiana Andrade
Etto, Rafael Mazer
Wohnrath, Karen
BIOSENSORS & BIOELECTRONICS, 2019, 141
[8] A label-free logic gate hairpin aptasensor for sensitive detection of ATP based on graphene oxide and PicoGreen dye
Zhang, Jingjing
Xu, Handan
Li, Chunhui
Wang, Yilin
Liu, Debing
Zhao, Shenghe
JOURNAL OF ANALYTICAL SCIENCE AND TECHNOLOGY, 2021, 12 (01)
[9] A label-free electrochemical immunosensor based on gold nanoparticles for detection of paraoxon
Hu, SQ
Xie, JW
Xu, QH
Rong, KT
Shen, GL
Yu, RQ
TALANTA, 2003, 61 (06) : 769 - 777
[10] A label-free logic gate hairpin aptasensor for sensitive detection of ATP based on graphene oxide and PicoGreen dye
Jingjing Zhang
Handan Xu
Chunhui Li
Yilin Wang
Debing Liu
Shenghe Zhao
Journal of Analytical Science and Technology, 12

← 1 2 3 4 5 →