A Label-Free Fluorescent DNA Calculator Based on Gold Nanoparticles for Sensitive Detection of ATP

被引：4

作者：

Zhang, Jingjing ^{[1
]}

Zhang, Shizhi ^{[1
]}

Niu, Chaoqun ^{[1
]}

Liu, Chen ^{[1
]}

Du, Jie ^{[1
]}

Chen, Yong ^{[1
]}

机构：

[1] Hainan Univ, Inst Trop Agr & Forestry, Coll Mat & Chem Engn,Coll Informat Sci & Technol, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Hainan, Peoples R China

来源：

MOLECULES | 2018年 / 23卷 / 10期

基金：

海南省自然科学基金;

关键词：

ATP detection; label-free fluorescence; enzyme-free; gold nanoparticles; DNA calculator; ADENOSINE 5'-TRIPHOSPHATE; SELECTIVE DETECTION; PROBE; SILVER; IONS; RECOVERY;

D O I：

10.3390/molecules23102494

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Herein we described a deoxyribonucleic acid (DNA) calculator for sensitive detection of the determination of adenosine triphosphate (ATP) using gold nanoparticles (GNP) and PicoGreen fluorescence dye as signal transducer, and ATP and single-stranded DNA (DNA-M') as activators. The calculator-related performances including linearity, reaction time, logic gate, and selectivity were investigated, respectively. The results revealed that this oligonucleotide sensor was highly sensitive and selective. The detection range was 50-500 nmol/L (R-2 = 0.99391) and the detection limit was 46.5 nmol/L. The AND DNA calculator was successfully used for the ATP detection in human urine. Compared with other methods, this DNA calculator has the characteristics of being label-free, non-enzymic, simple, and highly sensitive.

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页数：10

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