Fourier transform infrared spectroscopy reveals a rigid α-helical assembly for the tetrameric Streptomyces lividans K+ channel

被引：76
|
作者：
le Coutre, J
Kaback, HR
Patel, CKN
Heginbotham, L
Miller, C
机构：
[1] Univ Calif Los Angeles, Howard Hughes Med Inst, MacDonald Res Labs 6 720, Dept Physiol, Los Angeles, CA 90095 USA
[2] Univ Calif Los Angeles, Inst Mol Biol, Dept Microbiol & Mol Genet, Los Angeles, CA 90095 USA
[3] Univ Calif Los Angeles, Dept Phys & Astron, Los Angeles, CA 90095 USA
[4] Brandeis Univ, Howard Hughes Med Inst, Dept Biochem, Waltham, MA 02254 USA
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1998年 / 95卷 / 11期
关键词：
D O I：
10.1073/pnas.95.11.6114
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The structure of the tetrameric K+ channel from Streptomyces lividans in a lipid bilayer environment was studied by polarized attenuated total reflection Fourier transform infrared spectroscopy. The channel displays approximately 43% alpha-helical and 25% beta-sheet content. In addition, H/D exchange experiments show that only 43% of the backbone amide protons are exchangeable with solvent. On average, the alpha-helices are tilted 33 degrees normal to the membrane surface. The results are discussed in relationship to the lactose permease of Escherichia coli, a membrane transport protein.
引用
收藏
页码：6114 / 6117
页数：4
相关论文
共 50 条
  • [21] Fourier Transform Infrared Spectroscopy Reveals Molecular Changes in Blood Vessels of Rats Treated with Pentadecapeptide BPC 157
    Gamulin, Ozren
    Oroz, Katarina
    Coric, Luka
    Krajacic, Maria
    Skrabic, Marko
    Dretar, Vilim
    Strbe, Sanja
    Talapko, Jasminka
    Juzbasic, Martina
    Krezic, Ivan
    Lozic, Marin
    Stambolija, Vasilije
    Zizek, Helena
    Jurca, Ivana
    Jurjevic, Ivana
    Blagaic, Alenka Boban
    Skrtic, Anita
    Seiwerth, Sven
    Sikiric, Predrag
    BIOMEDICINES, 2022, 10 (12)
  • [22] Optical Analysis of Glioma: Fourier-Transform Infrared Spectroscopy Reveals the IDH1 Mutation Status
    Uckermann, Ortrud
    Juratli, Tareq A.
    Galli, Roberta
    Conde, Marina
    Wiedemuth, Ralf
    Krex, Dietmar
    Geiger, Kathrin
    Temme, Achim
    Schackert, Gabriele
    Koch, Edmund
    Steiner, Gerald
    Kirsch, Matthias
    CLINICAL CANCER RESEARCH, 2018, 24 (11) : 2530 - 2538
  • [23] Fourier transform infrared spectroscopy of the nu(2) vibration of BC2 in Ar at 10 K
    PresillaMarquez, JD
    Larson, CW
    Carrick, PG
    Rittby, CML
    JOURNAL OF CHEMICAL PHYSICS, 1996, 105 (09): : 3398 - 3405
  • [24] Inhibition and partial reactions of Na,K-ATPase studied by Fourier transform infrared difference spectroscopy
    Stolz, Michael
    Lewitzki, Erwin
    Maentele, Werner
    Barth, Andreas
    Grell, Ernst
    BIOPOLYMERS, 2006, 82 (04) : 368 - 372
  • [25] Interaction between Soluble and Membrane-Embedded Potassium Channel Peptides Monitored by Fourier Transform Infrared Spectroscopy
    Abbott, Geoffrey W.
    Ramesh, Bala
    Srai, Surjit K.
    PLOS ONE, 2012, 7 (11):
  • [26] Ion–protein interactions of a potassium ion channel studied by attenuated total reflection Fourier transform infrared spectroscopy
    Furutani Y.
    Biophysical Reviews, 2018, 10 (2) : 235 - 239
  • [27] Attenuated total reflection Fourier-transform infrared spectroscopy reveals environment specific phenotypes in clonal Japanese knotweed
    Holden, Claire A.
    Mcainsh, Martin
    Taylor, Jane E.
    Beckett, Paul
    Martin, Francis L.
    BMC PLANT BIOLOGY, 2024, 24 (01):
  • [28] Molecular orientation of rigid-rod polyimide films characterized by polarized attenuated total reflection/Fourier transform infrared spectroscopy
    Matsuda, SI
    Ando, SJ
    JOURNAL OF POLYMER SCIENCE PART B-POLYMER PHYSICS, 2003, 41 (04) : 418 - 428
  • [29] Macromolecular Changes in Nilotinib Resistant K562 Cells; an In vitro Study by Fourier Transform Infrared Spectroscopy
    Ceylan, Cagatay
    Camgoz, Aylin
    Baran, Yusuf
    TECHNOLOGY IN CANCER RESEARCH & TREATMENT, 2012, 11 (04) : 333 - 344
  • [30] Simultaneous measurement of B and P concentration in highly pure silicon by Fourier transform infrared spectroscopy at 4.2K
    Zhang, Jichang
    Wu, Jiangen
    Qu, Fenyuan
    Ye, Hongjuan
    Xiao, Jincai
    Yu, Zhiyi
    Lu, Wei
    Chinese Journal of Infrared Research, Series B (English Edition), 1988, 7 : 19 - 21
12345