Evaluation of capillary HPLC/mass spectrometry as an alternative analysis method for gel electrophoresis of oligonucleotides

被引：1

作者：

Rozenski, J ^{[1
]}

Vastmans, K ^{[1
]}

Van Aersehot, A ^{[1
]}

Herdewijn, P ^{[1
]}

机构：

[1] Katholieke Univ Leuven, Rega Inst Med Res, B-3000 Louvain, Belgium

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2003年 / 22卷 / 5-8期

关键词：

LC/MS; oligonucleotides; DNA polymers;

D O I：

10.1081/NCN-120023023

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method has been developed to monitor the enzymatic incorporation of nucleotides in DNA by electrospray HPLC mass spectrometry. The main advantages of mass spectrometry over electrophoresis are the ability to directly characterize the reaction products and the shorter analysis time.

引用

页码：1513 / 1516

页数：4