Direct observation of thiol ester formation between an enzyme and an active site directed inhibitor by FT-IR

被引：6

作者：

Kawasaki, T

Fujioka, Y

Saito, K

Ohta, H

机构：

[1] KEIO UNIV, DEPT CHEM, KOHOKU KU, YOKOHAMA, KANAGAWA 223, JAPAN

[2] NIPPON STEEL CORP LTD, ADV MAT & TECHNOL RES LABS, NAKAHARA KU, KAWASAKI, KANAGAWA 211, JAPAN

来源：

CHEMISTRY LETTERS | 1996年 / 03期

关键词：

D O I：

10.1246/cl.1996.195

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

FT-IR measurement disclosed a formation of a thiol ester bond between a cysteine of arylmalonate decarboxylase and an active site directed inhibitor, alpha-bromophenylacetate, strongly suggesting that the enzyme initially activates the substrates in a similar mechanism.

引用

页码：195 / 196

页数：2

共 7 条

[1]

BOYER PD, 1975, ENZYMES, V6

[2]

*INFR SPECTR ATL A, 1991, INFR SPECTR ATL COAT, V1, P502

[3] A NOVEL ENZYMATIC DECARBOXYLATION PROCEEDS VIA A THIOL ESTER INTERMEDIATE [J].