microRNA-217 suppressed epithelial-to-mesenchymal transition through targeting PTPN14 in gastric cancer (Publication with Expression of Concern. See vol. 40, 2020)

被引:19
作者
Chen, Gen [1 ]
Yang, Zhangshuo [2 ]
Feng, Maohui [2 ]
Wang, Zhiliang [3 ]
机构
[1] Gui Zhou Prov Peoples Hosp, Guiyang, Gui Zhou, Peoples R China
[2] Wuhan Univ, Zhongnan Hosp, Dept Gastroenterol Surg, Wuhan, Peoples R China
[3] Wuhan Univ, Zhongnan Hosp, Hubei Key Lab Med Technol Transplantat, Inst Hepatobiliary Dis,Transplant Ctr, Wuhan, Peoples R China
基金
美国国家科学基金会;
关键词
TUMOR-SUPPRESSOR; CARCINOMA; ZEB1;
D O I
10.1042/BSR20193176
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Background: Gastric cancer (GC) is the one of most common malignancies and its mechanism of metastasis remains unclear. The study was designed to investigate the effects of microRNA-217 on epithelial-to-mesenchymal transition. Methods: The expression levels of miR-217 in GC were assayed by real-time qPCR. Metastasis and invasion of cancer cell were assayed by transwell chamber. Double luciferase reporter gene was used to verify the target regulatory relationship between microRNA-217 and tyrosine-protein phosphatase non-receptor type 14 (PTPN14) on gastric cell lines. Epithelial-to-mesenchymal transition (EMT) markers were assayed by Western blot. Results: We found that miR-217 had a low level expression in gastric tumor tissues of 40 patients with GC, and a lower expression in the gastric tumor tissues of the patients with GC metastasis. Moreover, miR-217 markedly suppressed the metastasis and invasion of gastric cancer cell line in vitro. Furthermore, miR-217 inhibited the expression of PTPN14 by directly targeting its 3'UTR. Moreover, the down-regulation of PTPN14 reduced the metastasis and invasion, whereas up-regulation of PTPN14 led to the enhanced metastases and invasion of gastric cells. miR-217 induced the down-regulation of PTPN14 and inhibited the EMT in gastric cancer cells. Conclusion: miR-217 inhibited the EMT through directly targeting to the 3'UTR of PTPN14.
引用
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页数:9
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