Adaptation of the [3H]Hypoxanthine Uptake Assay for In Vitro-Cultured Plasmodium knowlesi Malaria Parasites

被引：14

作者：

Arnold, Megan S. J. ^{[1
]}

Engel, Jessica A. ^{[1
]}

Chua, Ming Jang ^{[1
]}

Fisher, Gillian M. ^{[1
]}

Skinner-Adams, Tina S. ^{[1
]}

Andrews, Katherine T. ^{[1
]}

机构：

[1] Griffith Univ, Eskitis Inst Drug Discovery, Nathan, Qld 4111, Australia

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2016年 / 60卷 / 07期

基金：

英国医学研究理事会;

关键词：

FALCIPARUM; INFECTIONS; INVITRO;

D O I：

10.1128/AAC.02948-15

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The zoonotic malaria parasite Plasmodium knowlesi has recently been established in continuous in vitro culture. Here, the Plasmodium falciparum [H-3]hypoxanthine uptake assay was adapted for P. knowlesi and used to determine the sensitivity of this parasite to chloroquine, cycloguanil, and clindamycin. The data demonstrate that P. knowlesi is sensitive to all drugs, with 50% inhibitory concentrations (IC(50)s) consistent with those obtained with P. falciparum. This assay provides a platform to use P. knowlesi in vitro for drug discovery.

引用

页码：4361 / 4363

页数：3

共 23 条

[11] MEASUREMENT OF THE LACTATE-DEHYDROGENASE ACTIVITY OF PLASMODIUM-FALCIPARUM AS AN ASSESSMENT OF PARASITEMIA [J].

MAKLER, MT ;

HINRICHS, DJ .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1993, 48 (02) :205-210

[12] Malaria surge feared [J].

Maxmen, Amy .

NATURE, 2012, 485 (7398) :293-293

[13] A Phase 3 Trial of RTS,S/AS01 Malaria Vaccine in African Infants [J].

Mian-McCarthy, Sara ;

Agnandji, Selidji Todagbe ;

Lell, Bertrand ;

Fernandes, Jose Francisco ;

Abossolo, Beatrice Peggy ;

Methogo, Barbara Gaelle Nfono Ondo ;

Kabwende, Anita Lumeka ;

Adegnika, Ayola Akim ;

Mordmueller, Benjamin ;

Issifou, Saadou ;

Kremsner, Peter Gottfried ;

Sacarlal, Jahit ;

Aide, Pedro ;

Lanaspa, Miguel ;

Aponte, John J. ;

Machevo, Sonia ;

Acacio, Sozinho ;

Bulo, Helder ;

Sigauque, Betuel ;

Macete, Eusebio ;

Alonso, Pedro ;

Abdulla, Salim ;

Salim, Nahya ;

Minja, Rose ;

Mpina, Maxmillian ;

Ahmed, Saumu ;

Ali, Ali Mohammed ;

Mtoro, Ali Takadir ;

Hamad, Ali Said ;

Mutani, Paul ;

Tanner, Marcel ;

Tinto, Halidou ;

D'Alessandro, Umberto ;

Sorgho, Hermann ;

Valea, Innocent ;

Bihoun, Biebo ;

Guiraud, Issa ;

Kabore, Berenger ;

Sombie, Olivier ;

Guiguemde, Robert Tinga ;

Ouedraogo, Jean Bosco ;

Hamel, Mary J. ;

Kariuki, Simon ;

Oneko, Martina ;

Odero, Chris ;

Otieno, Kephas ;

Awino, Norbert ;

McMorrow, Meredith ;

Muturi-Kioi, Vincent ;

Laserson, Kayla F. .

NEW ENGLAND JOURNAL OF MEDICINE, 2012, 367 (24) :2284-2295

[14] Adaptation of the genetically tractable malaria pathogen Plasmodium knowlesi to continuous culture in human erythrocytes [J].

Moon, Robert W. ;

Hall, Joanna ;

Rangkuti, Farania ;

Ho, Yung Shwen ;

Almond, Neil ;

Mitchell, Graham H. ;

Pain, Arnab ;

Holder, Anthony A. ;

Blackman, Michael J. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2013, 110 (02) :531-536

[15] Inhibitors of nonhousekeeping functions of the apicoplast defy delayed death in Plasmodium falciparum [J].

Ramya, T. N. C. ;

Mishra, Satyendra ;

Karmodiya, Krishanpal ;

Surolia, Namita ;

Surolia, Avadhesha .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2007, 51 (01) :307-316

[16] Improvement of culture conditions for long-term in vitro culture of Plasmodium vivax [J].

Roobsoong, Wanlapa ;

Tharinjaroen, Chayada S. ;

Rachaphaew, Nattawan ;

Chobson, Porpimon ;

Schofield, Louis ;

Cui, Liwang ;

Adams, John H. ;

Sattabongkot, Jetsumon .

MALARIA JOURNAL, 2015, 14

[17] P. falciparum In Vitro Killing Rates Allow to Discriminate between Different Antimalarial Mode-of-Action [J].

Sanz, Laura M. ;

Crespo, Benigno ;

De-Cozar, Cristina ;

Ding, Xavier C. ;

Llergo, Jose L. ;

Burrows, Jeremy N. ;

Garcia-Bustos, Jose F. ;

Gamo, Francisco-Javier .

PLOS ONE, 2012, 7 (02)

[18] Human Infections and Detection of Plasmodium knowlesi [J].

Singh, Balbir ;

Daneshvar, Cyrus .

CLINICAL MICROBIOLOGY REVIEWS, 2013, 26 (02) :165-184

[19] Simple and inexpensive fluorescence-based technique for high-throughput antimalarial drug screening [J].

Smilkstein, M ;

Sriwilaijaroen, N ;

Kelly, JX ;

Wilairat, P ;

Riscoe, M .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2004, 48 (05) :1803-1806

[20] Efficacy and safety of RTS,S/AS01 malaria vaccine with or without a booster dose in infants and children in Africa: final results of a phase 3, individually randomised, controlled trial [J].

Tinto, H. ;

D'Alessandro, U. ;

Sorgho, H. ;

Valea, I. ;

Tahita, M. C. ;

Kabore, W. ;

Kiemde, F. ;

Lompo, P. ;

Ouedraogo, S. ;

Derra, K. ;

Ouedraogo, F. ;

Ouedraogo, J. B. ;

Ballou, W. R. ;

Cohen, J. ;

Guerra, Y. ;

Heerwegh, D. ;

Jongert, E. ;

Lapierre, D. ;

Leach, A. ;

Lievens, M. ;

Ofori-Anyinam, O. ;

Olivier, A. ;

Vekemans, J. ;

Agnandji, S. T. ;

Lell, B. ;

Fernandes, J. F. ;

Abossolo, B. P. ;

Kabwende, A. L. ;

Adegnika, A. A. ;

Mordmueller, B. ;

Issifou, S. ;

Kremsner, P. G. ;

Loembe, M. M. ;

Bache, E. ;

Alabi, A. ;

Owusu-Agyei, S. ;

Asante, K. P. ;

Boahen, O. ;

Dosoo, D. ;

Asante, I. ;

Yidana, Z. ;

Anim, J. ;

Adeniji, E. ;

Yawson, A. K. ;

Kayan, K. ;

Chandramohan, D. ;

Greenwood, B. ;

Ansong, D. ;

Agbenyega, T. ;

Adjei, S. .

LANCET, 2015, 386 (9988) :31-45

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