Adaptation of the [3H]Hypoxanthine Uptake Assay for In Vitro-Cultured Plasmodium knowlesi Malaria Parasites

被引：14

作者：

Arnold, Megan S. J. ^{[1
]}

Engel, Jessica A. ^{[1
]}

Chua, Ming Jang ^{[1
]}

Fisher, Gillian M. ^{[1
]}

Skinner-Adams, Tina S. ^{[1
]}

Andrews, Katherine T. ^{[1
]}

机构：

[1] Griffith Univ, Eskitis Inst Drug Discovery, Nathan, Qld 4111, Australia

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2016年 / 60卷 / 07期

基金：

英国医学研究理事会;

关键词：

FALCIPARUM; INFECTIONS; INVITRO;

D O I：

10.1128/AAC.02948-15

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The zoonotic malaria parasite Plasmodium knowlesi has recently been established in continuous in vitro culture. Here, the Plasmodium falciparum [H-3]hypoxanthine uptake assay was adapted for P. knowlesi and used to determine the sensitivity of this parasite to chloroquine, cycloguanil, and clindamycin. The data demonstrate that P. knowlesi is sensitive to all drugs, with 50% inhibitory concentrations (IC(50)s) consistent with those obtained with P. falciparum. This assay provides a platform to use P. knowlesi in vitro for drug discovery.

引用

页码：4361 / 4363

页数：3

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