Ultrafast spectroscopy of biological photoreceptors

被引：89

作者：

Kennis, John T. M. ^{[1
]}

Groot, Marie-Louise ^{[1
]}

机构：

[1] Vrije Univ Amsterdam, Fac Sci, Dept Biophys, NL-1081 HV Amsterdam, Netherlands

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 2007年 / 17卷 / 05期

关键词：

D O I：

10.1016/j.sbi.2007.09.006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We review recent new insights on reaction dynamics of photoreceptors; proteins gained from ultrafast spectroscopy. In Blue Light sensing Using FAD (BLUF) domains, a hydrogenbond rearrangement around the flavin chromophore proceeds through a radical-pair mechanism, by which light-induced electron and proton transfer from the protein to flavin result in rotation of a conserved glutamine that switches the hydrogen bond network. Femtosecond infrared spectroscopy has shown that in photoactive yellow protein (PYP), breaking of a hydrogen bond that connects the p-coumaric acid chromophore to the backbone is crucial for trans-cis isomerization and successful entry into the photocycle. Furthermore, isomerization reactions of phycocyanobilin in phytochrome and retinal in the rhodopsins have been revealed in detail through application of femtosecond infrared and femtosecond-stimulated Raman spectroscopy.

引用

页码：623 / 630

页数：8

共 46 条

[1] Structure of a novel photoreceptor, the BLUF domain of AppA from Rhodobacter sphaeroides [J].

Anderson, S ;

Dragnea, V ;

Masuda, S ;

Ybe, J ;

Moffat, K ;

Bauer, C .

BIOCHEMISTRY, 2005, 44 (22) :7998-8005

[2] Proteorhodopsin phototrophy in the ocean [J].

Béjà, O ;

Spudich, EN ;

Spudich, JL ;

Leclerc, M ;

DeLong, EF .

NATURE, 2001, 411 (6839) :786-789

[3] Early molecular events in the photoactive yellow protein: role of the chromophore photophysics [J].

Changenet-Barret, P ;

Espagne, A ;

Charier, S ;

Baudin, JB ;

Jullien, L ;

Plaza, P ;

Hellingwerf, KJ ;

Martin, MM .

PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, 2004, 3 (08) :823-829

[4]

Christie JM, 2005, HANDBOOK OF PHOTOSENSORY RECEPTORS, P277, DOI 10.1002/352760510X.ch13

[5]

Crosson S, 2005, HANDBOOK OF PHOTOSENSORY RECEPTORS, P323, DOI 10.1002/352760510X.ch15

[6] Time-resolved spectroscopic studies of the AppA blue-light receptor BLUF domain from Rhodobacter sphaeroides [J].

Dragnea, V ;

Waegele, M ;

Balascuta, S ;

Bauer, C ;

Dragnea, B .

BIOCHEMISTRY, 2005, 44 (49) :15978-15985

[7] Photocycle of the flavin-binding photoreceptor AppA, a bacterial transcriptional antirepressor of photosynthesis genes [J].

Gauden, M ;

Yeremenko, S ;

Laan, W ;

van Stokkum, IHM ;

Ihalainen, JA ;

van Grondelle, R ;

Hellingwerf, KJ ;

Kennis, JTM .

BIOCHEMISTRY, 2005, 44 (10) :3653-3662

[8] On the role of aromatic side chains in the photoactivation of BLUF domains [J].

Gauden, Magdalena ;

Grinstead, Jeffrey S. ;

Laan, Wouter ;

van Stokkum, Ivo H. M. ;

Avila-Perez, Marcela ;

Toh, K. C. ;

Boelens, Rolf ;

Kaptein, Robert ;

van Grondelle, Rienk ;

Hellingwerf, Klaas J. ;

Kennis, John T. M. .

BIOCHEMISTRY, 2007, 46 (25) :7405-7415

[9] Hydrogen-bond switching through a radical pair mechanism in a flavin-binding photoreceptor [J].

Gauden, Magdalena ;

van Stokkum, Ivo H. M. ;

Key, Jason M. ;

Luehrs, Daniel Ch. ;

Van Grondelle, Rienk ;

Hegemann, Peter ;

Kennis, John T. M. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (29) :10895-10900

[10] BLUF: a novel FAD-binding domain involved in sensory transduction in microorganisms [J].

Gomelsky, M ;

Klug, G .

TRENDS IN BIOCHEMICAL SCIENCES, 2002, 27 (10) :497-500

← 1 2 3 4 5 →