Protective Effect of Lifor Solution in Experimental Renal Ischemia-Reperfusion Injury

Background Improved kidney preservation methods are needed to reduce ischemia reperfusion (IR) injury in kidney allografts Lifor is an artificial preservation solution comprised of nutrients, growth factors, and a non protein oxygen and nutrient carrier The current study compared the effectiveness of Lifor to University of Wisconsin solution (UW) in protecting rat kidneys from warm IR and cold storage injury Materials and Methods In a warm IR model, rat kidneys were perfused In situ with either saline, UW, or Lifor for 45 min Renal function and histology were assessed 24 h later In a cold IR model, kidney slices were cold stored in saline, UW, or Lifor at 4 degrees C Kidney injury was assessed by the release of lactate dehydrogenase (LDH) and immunoblot analysis for cleaved caspase 3 Results Lifor perfusion significantly mitigated renal dysfunction and tubular injury at 24 h compared with saline or UW Lifor and UW prevented LDH re lease in hypoxic kidney slices in vitro, however activation of caspase 3 following hypoxia reoxygenation was attenuated only with Lifor Cold storage with Lifor or UW significantly decreased LDH release from kidney slices or normal rat kidney cells in comparison to storage in saline or culture media After 24 h of cold storage there was a significant decrease in cleaved caspase 3 in Lifor stored slices compared that seen following cold storage in saline or UW solution Conclusions Lifor solution mitigates both warm and cold renal IR and appears to provide greater protection from apoptosis compared with UW solution (C) 2010 Elsevier Inc All rights Is served